Taxonomic group: bacteria / Firmicutes
(Phylum: Firmicutes)
Host organism: Bos taurus
Associated disease: mastitis [ICD11:
GB21.Z 
];
udder septicemia;
infection due to Streptococcus dysgalactiae [ICD11:
XN8UN ]
The structure was elucidated in this paperPublication DOI: 10.1016/j.carres.2013.12.018Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: kduda
fz-borstel.de
Institutions: Division of Structural Biochemistry, Priority Area of Asthma and Allergy, Research Center Borstel, Leibniz-Center for Medicine and Biosciences, Airway Research Center North (ARCN), Member of the German Center for Lung Research (DZL), Parkallee 4a/c, D-23845 Borstel, Germany
Different rhamnose-rich polysaccharides (RRP) were identified in the cell envelope of the Gram-positive bovine mastitis isolate Streptococcus dysgalactiae 2023. Structural investigations of the 1D and 2D nuclear magnetic resonance experiments as well as chemical analyses identified as main components l-Rha and d-GalNAc. Two main RRP were characterized, namely 1 being composed of the repeating unit {→3)-α-L-Rhap-(1→2)-[α-D-GalpNAc-(1→3)-β-D-GalpNAc-(1→3)-]α-l-Rhap-(1} and 2 possessing the repeat [→2)-α-L-Rhap-(1→3)-α-L-Rhap-(1→].
isolate, structural, polysaccharide, Streptococcus, repeating unit, cell, Research, chemical, polysaccharides, biochemistry, medicine, nuclear, nuclear magnetic resonance, resonance, component, NMR analysis, cell envelope, Gram-positive, rhamnan, bovine, lung, envelope, mastitis, Streptococcus dysgalactiae, allergy, Asthma
Structure type: polymer chemical repeating unit
Location inside paper: abstract, p.194, fig.3, 2
Trivial name: rhamnan
Compound class: CPS, cell wall polysaccharide
Contained glycoepitopes: IEDB_133754,IEDB_136105,IEDB_143254,IEDB_225177,IEDB_885823
Methods: 13C NMR, 1H NMR, methylation, GLC-MS, NMR-2D, acid hydrolysis, GLC, composition analysis, NMR-1D, acetylation
Comments, role: published polymerization frame was shifted for conformity with other records
Related record ID(s): 30107
NCBI Taxonomy refs (TaxIDs): 1334Reference(s) to other database(s): GTC:G05747CX, GlycomeDB:
5075
Show glycosyltransferases
NMR conditions: in D2O at 300 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
2 aLRhap 103.44 70.98 78.49 74.66 70.39 17.80
aLRhap 101.87 79.20 71.10 73.30 70.34 18.03
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
2 aLRhap 4.96 4.17 3.85 3.55 3.76 1.27
aLRhap 5.19 4.07 3.95 3.52 3.83 1.32
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
2 aLRhap 103.44/4.96 70.98/4.17 78.49/3.85 74.66/3.55 70.39/3.76 17.80/1.27
aLRhap 101.87/5.19 79.20/4.07 71.10/3.95 73.30/3.52 70.34/3.83 18.03/1.32
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 2 | aLRhap | 4.96 | 4.17 | 3.85 | 3.55 | 3.76 | 1.27 |
| | aLRhap | 5.19 | 4.07 | 3.95 | 3.52 | 3.83 | 1.32 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 2 | aLRhap | 103.44 | 70.98 | 78.49 | 74.66 | 70.39 | 17.80 |
| | aLRhap | 101.87 | 79.20 | 71.10 | 73.30 | 70.34 | 18.03 |
|
There is only one chemically distinct structure:
Found 
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