Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Host organism: Homo sapiens
Associated disease: urinary tract infections (UTI) [ICD11:
GC08 
];
infectious gastroenteritis [ICD11:
1A40.Z ]
The structure was elucidated in this paperNCBI PubMed ID: 25240185Publication DOI: 10.1016/j.carres.2014.07.008Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: yknirel
gmail.com (Y.A. Knirel)
Institutions: Department of Immunobiology of Bacteria, Institute of Microbiology, Biotechnology and Immunology, University of Lodz, PL 90-237 Lodz, Poland, TEDA School of Biological Sciences and Biotechnology, Nankai University, TEDA, 300457 Tianjin, PR China, N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia
O-Polysaccharide was obtained by mild acid degradation of the lipopolysaccharide of Providencia alcalifaciens O45:H25 and studied by sugar analysis, Smith degradation, and (1)H and (13)C NMR spectroscopy. The following structure of the pentasaccharide repeat of the O-polysaccharide was established: [structure: see text]. The O-antigen gene cluster of P. alcalifaciens O45 was sequenced and found to be in full agreement with the O-polysaccharide structure established.
Lipopolysaccharide, structure, gene, O-antigen, O antigen, cluster, gene cluster, O-polysaccharide, O polysaccharide, Providencia, Providencia alcalifaciens, bacterial polysaccharide structure, organization, O-antigen gene cluster
Structure type: polymer chemical repeating unit
Location inside paper: p.75, PS-2
Compound class: O-polysaccharide
Contained glycoepitopes: IEDB_115136,IEDB_135813,IEDB_136045,IEDB_137340,IEDB_140630,IEDB_141807,IEDB_142488,IEDB_142489,IEDB_144562,IEDB_145669,IEDB_146664,IEDB_150092,IEDB_151531,IEDB_152214,IEDB_174333,IEDB_490056,IEDB_983931,SB_192,SB_86
Methods: 13C NMR, 1H NMR, GLC-MS, NMR-2D, DNA sequencing, sugar analysis, GLC, mild acid hydrolysis, Smith degradation, de-O-acetylation, NMR-1D, GPC, function analysis of gene clusters
Comments, role: O-deacetylated polysaccharide from P. alcalifaciens O45:H25.
Related record ID(s): 30110, 30420
NCBI Taxonomy refs (TaxIDs): 126385Reference(s) to other database(s): GTC:G45291GY
Show glycosyltransferases
NMR conditions: in D2O at 303 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
3,3,3 aLFucp 100.4 68.0 78.5 70.7 68.0 16.7
3,3 aDGlcpA 102.0 73.5 80.8 72.0 75.2 177.5
3 aLFucp 100.3 68.2 78.5 73.3 67.7 16.6
2 Ac 175.8 23.6
4 bDGlcp 102.7 75.0 76.8 71.9 77.6 63.5
bDGlcpN 100.1 57.4 76.6 74.9 76.7 61.1
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
3,3,3 aLFucp 5.29 3.90 4.07 3.89 4.33 1.20
3,3 aDGlcpA 5.18 3.80 3.88 3.62 4.07 -
3 aLFucp 5.12 3.92 3.87 3.90 4.80 1.18
2 Ac - 2.03
4 bDGlcp 4.51 3.22 3.49 3.13 3.42 3.60-3.89
bDGlcpN 4.73 3.93 3.93 3.96 3.59 3.87-4.01
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
3,3,3 aLFucp 100.4/5.29 68.0/3.90 78.5/4.07 70.7/3.89 68.0/4.33 16.7/1.20
3,3 aDGlcpA 102.0/5.18 73.5/3.80 80.8/3.88 72.0/3.62 75.2/4.07
3 aLFucp 100.3/5.12 68.2/3.92 78.5/3.87 73.3/3.90 67.7/4.80 16.6/1.18
2 Ac 23.6/2.03
4 bDGlcp 102.7/4.51 75.0/3.22 76.8/3.49 71.9/3.13 77.6/3.42 63.5/3.60-3.89
bDGlcpN 100.1/4.73 57.4/3.93 76.6/3.93 74.9/3.96 76.7/3.59 61.1/3.87-4.01
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 3,3,3 | aLFucp | 5.29 | 3.90 | 4.07 | 3.89 | 4.33 | 1.20 |
| 3,3 | aDGlcpA | 5.18 | 3.80 | 3.88 | 3.62 | 4.07 |
|
| 3 | aLFucp | 5.12 | 3.92 | 3.87 | 3.90 | 4.80 | 1.18 |
| 2 | Ac |
| 2.03 | |
| 4 | bDGlcp | 4.51 | 3.22 | 3.49 | 3.13 | 3.42 | 3.60
3.89 |
| | bDGlcpN | 4.73 | 3.93 | 3.93 | 3.96 | 3.59 | 3.87
4.01 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 3,3,3 | aLFucp | 100.4 | 68.0 | 78.5 | 70.7 | 68.0 | 16.7 |
| 3,3 | aDGlcpA | 102.0 | 73.5 | 80.8 | 72.0 | 75.2 | 177.5 |
| 3 | aLFucp | 100.3 | 68.2 | 78.5 | 73.3 | 67.7 | 16.6 |
| 2 | Ac | 175.8 | 23.6 | |
| 4 | bDGlcp | 102.7 | 75.0 | 76.8 | 71.9 | 77.6 | 63.5 |
| | bDGlcpN | 100.1 | 57.4 | 76.6 | 74.9 | 76.7 | 61.1 |
|
There is only one chemically distinct structure:
report error
Found 1 record.
Displayed record 1
