Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Host organism: Homo sapiens
Associated disease: urinary tract infections (UTI) [ICD11:
GC08 
];
infectious gastroenteritis [ICD11:
1A40.Z ]
The structure was elucidated in this paperNCBI PubMed ID: 25240185Publication DOI: 10.1016/j.carres.2014.07.008Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: yknirel
gmail.com (Y.A. Knirel)
Institutions: Department of Immunobiology of Bacteria, Institute of Microbiology, Biotechnology and Immunology, University of Lodz, PL 90-237 Lodz, Poland, TEDA School of Biological Sciences and Biotechnology, Nankai University, TEDA, 300457 Tianjin, PR China, N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia
O-Polysaccharide was obtained by mild acid degradation of the lipopolysaccharide of Providencia alcalifaciens O45:H25 and studied by sugar analysis, Smith degradation, and (1)H and (13)C NMR spectroscopy. The following structure of the pentasaccharide repeat of the O-polysaccharide was established: [structure: see text]. The O-antigen gene cluster of P. alcalifaciens O45 was sequenced and found to be in full agreement with the O-polysaccharide structure established.
Lipopolysaccharide, structure, gene, O-antigen, O antigen, cluster, gene cluster, O-polysaccharide, O polysaccharide, Providencia, Providencia alcalifaciens, bacterial polysaccharide structure, organization, O-antigen gene cluster
Structure type: polymer chemical repeating unit
Location inside paper: p.75, PS-3
Compound class: O-polysaccharide
Contained glycoepitopes: IEDB_115136,IEDB_135813,IEDB_136045,IEDB_137340,IEDB_140630,IEDB_141807,IEDB_142489,IEDB_144562,IEDB_145669,IEDB_150092,IEDB_151531,IEDB_152214,IEDB_174333,IEDB_490056,SB_86
Methods: 13C NMR, 1H NMR, GLC-MS, NMR-2D, DNA sequencing, sugar analysis, GLC, mild acid hydrolysis, Smith degradation, de-O-acetylation, NMR-1D, GPC, function analysis of gene clusters
Comments, role: Smith-degraded polysaccharide from P. alcalifaciens O45:H25.
Related record ID(s): 30110, 30419
NCBI Taxonomy refs (TaxIDs): 126385Reference(s) to other database(s): GTC:G16185PO
Show glycosyltransferases
NMR conditions: in D2O at 303 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
3,3,3 aLFucp 100.4 68.0 78.5 70.7 68.1 16.7
3,3 aDGlcpA 101.9 73.5 80.5 71.8 74.2 176.9
3 aLFucp 101.2 68.6 79.0 73.2 68.3 16.5
2 Ac 176.2 23.7
bDGlcpN 100.1 56.9 81.3 69.9 77.3 62.2
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
3,3,3 aLFucp 5.28 3.87 4.07 3.89 4.34 1.18
3,3 aDGlcpA 5.19 3.77 3.87 3.62 4.14 -
3 aLFucp 5.01 3.90 3.91 3.92 4.34 1.14
2 Ac - 2.01
bDGlcpN 4.68 3.86 3.71 3.53 3.45 3.75-3.93
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
3,3,3 aLFucp 100.4/5.28 68.0/3.87 78.5/4.07 70.7/3.89 68.1/4.34 16.7/1.18
3,3 aDGlcpA 101.9/5.19 73.5/3.77 80.5/3.87 71.8/3.62 74.2/4.14
3 aLFucp 101.2/5.01 68.6/3.90 79.0/3.91 73.2/3.92 68.3/4.34 16.5/1.14
2 Ac 23.7/2.01
bDGlcpN 100.1/4.68 56.9/3.86 81.3/3.71 69.9/3.53 77.3/3.45 62.2/3.75-3.93
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 3,3,3 | aLFucp | 5.28 | 3.87 | 4.07 | 3.89 | 4.34 | 1.18 |
| 3,3 | aDGlcpA | 5.19 | 3.77 | 3.87 | 3.62 | 4.14 |
|
| 3 | aLFucp | 5.01 | 3.90 | 3.91 | 3.92 | 4.34 | 1.14 |
| 2 | Ac |
| 2.01 | |
| | bDGlcpN | 4.68 | 3.86 | 3.71 | 3.53 | 3.45 | 3.75
3.93 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 3,3,3 | aLFucp | 100.4 | 68.0 | 78.5 | 70.7 | 68.1 | 16.7 |
| 3,3 | aDGlcpA | 101.9 | 73.5 | 80.5 | 71.8 | 74.2 | 176.9 |
| 3 | aLFucp | 101.2 | 68.6 | 79.0 | 73.2 | 68.3 | 16.5 |
| 2 | Ac | 176.2 | 23.7 | |
| | bDGlcpN | 100.1 | 56.9 | 81.3 | 69.9 | 77.3 | 62.2 |
|
There is only one chemically distinct structure:
Found 
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