Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Host organism: Homo sapiens
Associated disease: neonatal necrotising enterocolitis [ICD11:
KB88 
, Life stage: neonatal];
bacteremia [ICD11:
MA15.0 ];
septicemia [ICD11:
MA15.Y ];
meningitis [ICD11:
1D01 ]
The structure was elucidated in this paperPublication DOI: 10.1007/s11172-015-0999-yJournal NLM ID: 100912060Publisher: New York: Consultants Bureau
Correspondence: yknirel
gmail.com (Y.A. Knirel)
Institutions: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, TEDA Institute of Biological Sciences and Biotechnology, Nankai University, Tianjin Economic-Technological Development Area, Tianjin, 300457, People´s Republic of China, Key Laboratory of Molecular Microbiology and Technology of the Ministry of Education, College of Life Sciences, Nankai University, Tianjin, 300071, People’s Republic of China
Partially O-acetylated fructan was isolated by phenol—water extraction of cells of Cronobacter dublinensis G2732 followed by anion-exchange chromatography on DEAE-Toyopearl 650M. Structure of the isolated fructan was established by 2D 1H and 13C NMR spectroscopy. The same fructan but lacking O-acetyl groups was isolated from two other C. dublinensis strains, G3947 and G4061. Genes for the fructan biosynthesis were not found at the typical Cronobacter O-antigen gene cluster located between the conserved genes galF and gnd and are evidently located elsewhere on the chromosome.
Lipopolysaccharide, bacterial polysaccharide structure, fructan, Cronobacter dublinensis
Structure type: polymer chemical repeating unit
Location inside paper: p.1194, table 1
Compound class: O-polysaccharide
Methods: 13C NMR, 1H NMR, NMR-2D, sugar analysis, mild acid hydrolysis, GPC
Related record ID(s): 30914
NCBI Taxonomy refs (TaxIDs): 413497Reference(s) to other database(s): GTC:G93491BA
Show glycosyltransferases
NMR conditions: in D2O at 303 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
1,4 50%Ac ? 21.7
1 aDFruf 59.0 110.3 79.3 81.5 84.9 62.9
bDFruf 61.1 104.5 77.6 75.2 82.5 63.3
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
1,4 50%Ac - 2.19
1 aDFruf 3.77-3.98 - 4.21 4.90 4.21 3.78-3.86
bDFruf 3.76-3.87 - 4.37 4.21 3.88 3.73-3.87
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
1,4 50%Ac 21.7/2.19
1 aDFruf 59.0/3.77-3.98 79.3/4.21 81.5/4.90 84.9/4.21 62.9/3.78-3.86
bDFruf 61.1/3.76-3.87 77.6/4.37 75.2/4.21 82.5/3.88 63.3/3.73-3.87
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 1,4 | 50%Ac |
| 2.19 | |
| 1 | aDFruf | 3.77
3.98 |
| 4.21 | 4.90 | 4.21 | 3.78
3.86 |
| | bDFruf | 3.76
3.87 |
| 4.37 | 4.21 | 3.88 | 3.73
3.87 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 1,4 | 50%Ac | ? | 21.7 | |
| 1 | aDFruf | 59.0 | 110.3 | 79.3 | 81.5 | 84.9 | 62.9 |
| | bDFruf | 61.1 | 104.5 | 77.6 | 75.2 | 82.5 | 63.3 |
|
![](nmr_drawer.py?width=750&height=130&nucleus=C&rotate_labels=false&spectrum=[{"s":21.7,"l":"2","c":[0,0,225]},{"s":59,"l":"1","c":[230,0,30]},{"s":110.3,"l":"2","c":[230,0,30]},{"s":79.3,"l":"3","c":[230,0,30]},{"s":81.5,"l":"4","c":[230,0,30]},{"s":84.9,"l":"5","c":[230,0,30]},{"s":62.9,"l":"6","c":[230,0,30]},{"s":61.1,"l":"1","c":[0,165,0]},{"s":104.5,"l":"2","c":[0,165,0]},{"s":77.6,"l":"3","c":[0,165,0]},{"s":75.2,"l":"4","c":[0,165,0]},{"s":82.5,"l":"5","c":[0,165,0]},{"s":63.3,"l":"6","c":[0,165,0]}])
The spectrum also has 1 signal at unknown position (not plotted). |
There is only one chemically distinct structure:
Found 
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(previously named: Enterobacter sakazakii G2732)