Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Escherichia coli [ICD11:
XN6P4 
]
NCBI PubMed ID: 25439943Publication DOI: 10.1016/j.carbpol.2014.08.119Journal NLM ID: 8307156Publisher: Elsevier
Correspondence: jhchenwhut
126.com
Institutions: Wuxi Medical School, Jiangnan University, Wuxi 214122, PR China, Changshan Biochem Pharm (Jiangsu) Co., Ltd., Changzhou 213149, PR China, The Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, PR China, School of Pharmaceutical Science, Jiangnan University, Wuxi 214122, PR China
Escherichia coli K5 capsular polysaccharide (K5PS) has been used as starting material to synthesize heparan sulfate (HS) mimetics with various biological properties. This study determined the immunomodulatory activities of K5PS through the sulfation process. The immunomodulatory effects of sulfated K5 polysaccharide derivatives were evaluated in vitro on murine macrophages and lymphocytes. Results indicated that HS mimetics with high sulfation in the O-position stimulated cell proliferation of macrophages and lymphocytes, significantly enhanced cytokine secretion and upregulated the cytotoxicity of NK cells. This study suggests that high sulfation in O-position of HS is required for the immunomodulatory activities.
Escherichia coli, sulfation, sulfate, immunomodulatory activity, K5 capsular polysaccharide, Structure-activity correlation
Structure type: polymer chemical repeating unit
Location inside paper: p.644, fig.1, p.649
Trivial name: K5 polysaccharide, K-antigen, N-acetyl heparosan, heparosan (N-acetylheparosan), heparosan, heparosan (glycosaminoglycan GAG), K5 CPS, heparosan (K5-antigen), N-acetylheparosan
Compound class: CPS, EPS, K-antigen, polysaccharide
Contained glycoepitopes: IEDB_115136,IEDB_137340,IEDB_140630,IEDB_141807,IEDB_151531,IEDB_153764,IEDB_423153
Methods: 13C NMR, ELISA, chemical synthesis, biological assays, statistical analysis, immunological assays
Biological activity: evaluation the immunoregulatory activity in vitro of sulfated K5 polysaccharide derivatives
NCBI Taxonomy refs (TaxIDs): 1010798Reference(s) to other database(s): GTC:G26089XS, GlycomeDB:
656
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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