Human Health Therapeutics Portfolio, National Research Council of Canada, 100 Sussex Drive, Ottawa, ON K1A 0R6, Canada, Public Health Agency of Canada, Toronto, ON, Canada
The core region of the lipopolysaccharide of Morganella morganii serotype O:1ab was obtained by hydrolysis of the LPS and studied by 2D NMR, ESI MS, and chemical methods. Its structure was highly homologous to those from the two major members of the same Proteeae tribe, Proteus mirabilis and Providencia alcalifaciens, and analysis of the M. morganii genome disclosed that the loci for its outer core, lipid A and Ara4N moieties are similarly conserved.
13C NMR, 1H NMR, methylation, NMR-2D, DNA sequencing, GC-MS, de-O-acylation, sugar analysis, dephosphorylation, 31P NMR, ESI-MS, acid hydrolysis, HPAEC, GC, deamination, GPC, UV
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6 C7
5,3,2,6,0 xXEtN 63.1 41.1
5,3,2,6 P
5,3,2 bDGlcp 104.4 73.8 75.8 69.3 75.8 64.8
5,3,3,2 aXLDmanHepp 97.9 71.0 71.6 67.0 72.7 69.8 63.8
5,3,3,4,3,6 aDGalp 99.2 69.3 70.4 70.3 71.9 62.2
5,3,3,4,3 bDGlcp 104.6 74.0 76.5 70.2 75.3 66.7
5,3,3,4,6,0 xXEtN 63.0 41.1
5,3,3,4,6 P
5,3,3,4 aDGalpN 96.8 51.6 78.5 67.3 70.0 64.3
5,3,3 aDGalpA 98.1 71.9 67.8 79.6 71.8 ?
5,3,7 aXLDmanHepp 102.2 71.0 71.6 67.0 72.2 69.8 ?
5,3 aXLDmanHepp ? 79.2 75.6 67.8 73.7 67.6 69.9
5,4 bDGlcp 103.3 74.7 76.7 71.4 77.1 62.1
5 aXLDmanHepp ? 71.1 79.2 72.1 72.3 69.2 63.7
8 bLArap4N 99.5 68.9 66.5 52.9 59.1
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1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6 H7
5,3,2,6,0 xXEtN 4.19 3.31
5,3,2,6 P
5,3,2 bDGlcp 4.47 3.23 3.50 3.60 3.50 4.19-4.26
5,3,3,2 aXLDmanHepp 5.13 3.98 3.87 3.87 3.87 4.04 3.73
5,3,3,4,3,6 aDGalp 5.01 3.85 3.88 4.01 3.96 3.75-3.75
5,3,3,4,3 bDGlcp 4.74 3.41 3.56 3.56 3.70 3.78-4.00
5,3,3,4,6,0 xXEtN 4.11 3.31
5,3,3,4,6 P
5,3,3,4 aDGalpN 5.28 3.73 4.22 4.44 4.70 3.96-4.00
5,3,3 aDGalpA 5.64 4.03 4.19 4.50 4.53 -
5,3,7 aXLDmanHepp 4.94 3.99 3.87 3.87 3.63 4.07 ?
5,3 aXLDmanHepp 5.37 4.10 4.14 4.04 3.60 4.24 3.66-3.76
5,4 bDGlcp 4.54 3.37 3.53 3.37 3.44 3.65-3.89
5 aXLDmanHepp 5.13 3.99 4.08 4.34 3.79 4.07 3.75-3.75
8 bLArap4N 5.02 3.76 4.18 3.72 3.80-4.13
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1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6 C7/H7
5,3,2,6,0 xXEtN 63.1/4.19 41.1/3.31
5,3,2,6 P
5,3,2 bDGlcp 104.4/4.47 73.8/3.23 75.8/3.50 69.3/3.60 75.8/3.50 64.8/4.19-4.26
5,3,3,2 aXLDmanHepp 97.9/5.13 71.0/3.98 71.6/3.87 67.0/3.87 72.7/3.87 69.8/4.04 63.8/3.73
5,3,3,4,3,6 aDGalp 99.2/5.01 69.3/3.85 70.4/3.88 70.3/4.01 71.9/3.96 62.2/3.75-3.75
5,3,3,4,3 bDGlcp 104.6/4.74 74.0/3.41 76.5/3.56 70.2/3.56 75.3/3.70 66.7/3.78-4.00
5,3,3,4,6,0 xXEtN 63.0/4.11 41.1/3.31
5,3,3,4,6 P
5,3,3,4 aDGalpN 96.8/5.28 51.6/3.73 78.5/4.22 67.3/4.44 70.0/4.70 64.3/3.96-4.00
5,3,3 aDGalpA 98.1/5.64 71.9/4.03 67.8/4.19 79.6/4.50 71.8/4.53
5,3,7 aXLDmanHepp 102.2/4.94 71.0/3.99 71.6/3.87 67.0/3.87 72.2/3.63 69.8/4.07 ?/?
5,3 aXLDmanHepp ?/5.37 79.2/4.10 75.6/4.14 67.8/4.04 73.7/3.60 67.6/4.24 69.9/3.66-3.76
5,4 bDGlcp 103.3/4.54 74.7/3.37 76.7/3.53 71.4/3.37 77.1/3.44 62.1/3.65-3.89
5 aXLDmanHepp ?/5.13 71.1/3.99 79.2/4.08 72.1/4.34 72.3/3.79 69.2/4.07 63.7/3.75-3.75
8 bLArap4N 99.5/5.02 68.9/3.76 66.5/4.18 52.9/3.72 59.1/3.80-4.13
?XKdop
report error
Found 1 record.
Displayed record 1
]; infection due to Morganella [ICD11: XN8G6 
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