Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: gastroenteritis [ICD11:
1A40.0 
];
enterocolitis [ICD11:
1A40.Z ];
acute inflammatory demyelinating polyneuropathy [ICD11:
8C01.0 ];
autoimmune reactions [ICD11:
4A01.21 , ICD11:
4A4Z ];
infection due to Campylobacter jejuni [ICD11:
XN4Q5 ]
NCBI PubMed ID: 25766665Publication DOI: 10.1111/mmi.12995Journal NLM ID: 8712028Publisher: Blackwell Publishing
Correspondence: ccreuzen
uwo.ca
Institutions: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, Department of Microbiology and Immunology, Western University, DSB 3031, London, ON, N6A 5C1, Canada, INRS-Institut Armand Frappier, Laval, QC, Canada, Centre for Infection and Immunity, Queen's University of Belfast, Belfast, UK
The Campylobacter jejuni capsular polysaccharide is important for virulence and often contains a modified heptose. In strain ATCC 700819 (a.k.a. NCTC 11168), the modified heptose branches off from the capsular backbone and is directly exposed to the environment. We reported previously that the enzymes encoded by wcaG, mlghB and mlghC are involved in heptose modification. Here, we show that inactivation of any of these genes leads to production of capsule lacking modified heptose and alters the transcription of other capsule modification genes differentially. Inactivation of mlghB or mlghC, but not of wcaG, decreased susceptibility to bile salts and abrogated invasion of intestinal cells. All mutants showed increased sensitivity to serum killing, especially wcaG::cat, and had defects in colonization and persistence in chicken intestine, but did not show significant differences in adhesion, phagocytosis and intracellular survival in murine macrophages. Together, our findings suggest that the capsular heptose modification pathway contributes to bacterial resistance against gastrointestinal host defenses and supports bacterial persistence via its role in serum resistance and invasion of intestinal cells. Our data further suggest a dynamic regulation of expression of this pathway in the gastrointestinal tract.
capsular polysaccharide, Campylobacter jejuni, capsule modification genes
Structure type: polymer chemical repeating unit
Location inside paper: p.1137, fig.1A
Compound class: CPS
Contained glycoepitopes: IEDB_115136,IEDB_120354,IEDB_137473,IEDB_140630,IEDB_149136
Methods: 13C NMR, 1H NMR, NMR-2D, SDS-PAGE, sugar analysis, DNA techniques, 31P NMR, acid hydrolysis, Western blotting, NMR-1D, serological methods, genetic methods, GPC, Southern blotting, RT-PCR, mutation analysis
Biological activity: serological data
Biosynthesis and genetic data: genetic data
Comments, role: Schematic representation of the CPS from C. jejuni ATCC 700819 (NCTC 1168).
Related record ID(s): 30894, 30895, 30896
NCBI Taxonomy refs (TaxIDs): 197
Show glycosyltransferases
There is only one chemically distinct structure:
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