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1. (Article ID: 3078)
 
Kai A, Arashida T, Hatanaka K, Akaike T, Matsuzaki K, Mimura T, Kaneko Y
Analysis of the biosynthetic process of cellulose and curdlan using 13C-labeled glucoses
Carbohydrate Polymers 23 (1994) 235-239
 

In order to elucidate the biosynthetic process of cellulose and curdlan, 13Clabeled polysaccharides were biosynthesized by Acetobacter xylinum (IFO 13693) and Agrobacterium sp. (ATCC 317491, from culture media containing D-(1-13C)glucose, D-(2-13C)glucose, D-(4-13C)glucose, or D-(6-13C)glucose as the carbon source, and their structures were determined by 13C NMR spectroscopy. The labeling was mainly found in the original position, indicating direct polymerization of introduced glucoses. In addition, the transfer of labeling from C-2 to C-I, C-3 and C-5, from C-4 to C-l, C-2 and C-3, and from C-6 to C-1 was found in celluloses. In curdlan, the transfer of labeling from C-1 to C-3, from C-2 to C-I and C-3, from C-4 to C-I, C-2 and C-3, and from C-6 to C-I and C-3 was observed. From analysis of this labeling, the biosynthetic process of cellulose and curdlan was explained as involving six routes. The percentages of each route via which cellulose or curdlan is biosynthesized were estimated for upper (C-t to C-3) and lower portions (C-4 to C-6) of glucosidic units in the polysaccharides. It is noted that very few polysaccharides are formed via the Embden-Meyerhof pathway. The lower half (C-4 to C-6) structure of introduced glucoses is well preserved in the polysaccharides.

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