Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Host organism: Homo sapiens
Associated disease: meningitis [ICD11:
1D01 
];
neonatal necrotising enterocolitis [ICD11:
KB88 , Life stage: neonatal]
NCBI PubMed ID: 25723620Publication DOI: 10.1016/j.carres.2015.01.020Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: zbigniew.kaczynski
ug.edu.pl (Z. Kaczyński)
Institutions: Faculty of Chemistry, University of Gdansk, Gdansk, Poland, School of Science and Technology, Nottingham Trent University, Clifton Lane, Nottingham NG11 8NS, United Kingdom, Department of Medical Microbiology, Medical University of Gdansk, Do Studzienki 38, 80-227 Gdansk, Poland
The O-polysaccharide (OPS) of Cronobacter sakazakii NTU 696 (Sequence Type 12) from a case of neonatal necrotizing enterocolitis was isolated from the polysaccharide fraction obtained after lipopolysaccharide (LPS) hydrolysis. Purified OPS was analyzed by NMR spectroscopy ((1)H, COSY, TOCSY, NOESY, HSQC, HSQC-TOCSY and HMBC experiments) and chemical methods. Obtained monosaccharide derivatives analyzed by gas chromatography and gas chromatography-mass spectrometry allowed the identification of six sugar components. Performed experiments enabled to establish a structure of the OPS repeating unit of C. sakazakii NTU 696, as: [structure: see text].
NMR, structure, O-polysaccharide, Cronobacter sakazakii
Structure type: polymer chemical repeating unit
Location inside paper: p.57
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_130648,IEDB_135813,IEDB_137340,IEDB_137473,IEDB_1391961,IEDB_140629,IEDB_141584,IEDB_141807,IEDB_142488,IEDB_144998,IEDB_146664,IEDB_151531,IEDB_423115,IEDB_885822,IEDB_983931,SB_192
Methods: 13C NMR, 1H NMR, methylation, GLC-MS, NMR-2D, GC-MS, acid hydrolysis, GLC, composition analysis, NMR-1D, GPC
Comments, role: Cronobacter sakazakii G2594 (serotype O4); published polymerization frame was shifted for conformity with other records.
Related record ID(s): 30634
NCBI Taxonomy refs (TaxIDs): 28141
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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