Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Host organism: Homo sapiens
Associated disease: infection due to Enterobacter cloacae [ICD11:
XN3YM 
]
Publication DOI: 10.1016/j.carres.2017.02.006Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: perepel
ioc.ac.ru (A. V. Perepelov)
Institutions: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, TEDA Institute of Biological Sciences and Biotechnology, Nankai University, TEDA, Tianjin, China, Key Laboratory of Molecular Microbiology and Technology of the Ministry of Education, College of Life Sciences, Nankai University, Tianjin, China, Tianjin Biochip Corporation, TEDA, Tianjin, China
On mild acid degradation of the lipopolysaccharide of Enterobacter cloacae C5529, the O-polysaccharide chain was cleaved at the linkages of 5,7-diacetamido-3,5,7,9-tetradeoxy-L-glycero-L-manno-non 2 ulosonic acid (di-N-acetylpseudaminic acid, Pse5Ac7Ac). The resultant oligosaccharide and an alkali-treated lipopolysaccharide were studied by sugar analysis along with 1H and 13C NMR spectroscopy, and the following structure of the tetrasaccharide repeating unit of the O-polysaccharide was established: [structure, see text].
O-antigen, pseudaminic acid, bacterial polysaccharide structure, O-antigen gene cluster, Enterobacter cloacae
Structure type: polymer chemical repeating unit
Location inside paper: chart 1
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_136044,IEDB_136095,IEDB_136906,IEDB_137472,IEDB_141794,IEDB_151528,IEDB_190606,SB_165,SB_166,SB_187,SB_195,SB_7,SB_88
Methods: 13C NMR, 1H NMR, NMR-2D, PCR, de-O-acylation, sugar analysis, DNA techniques, acid hydrolysis, GLC, mild acid hydrolysis, NMR-1D, GPC, function analysis of gene clusters
Biosynthesis and genetic data: genetic data
Related record ID(s): 30650, 30830
NCBI Taxonomy refs (TaxIDs): 550Reference(s) to other database(s): GTC:G27149NC
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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