Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Escherichia coli [ICD11:
XN6P4 
]
The structure was elucidated in this paperNCBI PubMed ID: 26342864Publication DOI: 10.1016/j.carres.2015.08.008Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: perepel
ioc.ac.ru (A. V. Perepelov)
Institutions: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, TEDA Institute of Biological Sciences and Biotechnology, TEDA, Nankai University, 300457 Tianjin, China
The O-polysaccharide (O-antigen) of Escherichia coli O43 was isolated from the lipopolysaccharide and studied by chemical methods, including sugar analyses, Smith degradation, and solvolysis with anhydrous trifluoroacetic acid, along with (1)H and (13)C NMR spectroscopy. The following structure of the pentasaccharide repeating unit of the O-polysaccharide was established: Functions of genes in the O-antigen gene cluster of E. coli O43 were assigned by a comparison with sequences in the available databases and found to be in agreement with the O-polysaccharide structure.
Lipopolysaccharide, O-antigen, Escherichia coli, bacterial polysaccharide structure, O-antigen gene cluster
Structure type: polymer chemical repeating unit
Location inside paper: p.33, p.34, table 1, Smith degraded polysaccharide
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_130648,IEDB_130701,IEDB_136045,IEDB_137473,IEDB_137485,IEDB_142489,IEDB_144562,IEDB_144983,IEDB_152206,IEDB_152214,IEDB_174333,IEDB_983930,SB_44,SB_67,SB_72,SB_86
Methods: 13C NMR, 1H NMR, NMR-2D, de-O-acylation, sugar analysis, GLC, mild acid hydrolysis, Smith degradation, NMR-1D, GPC, bioinformatic analysis, solvolysis with trifluoroacetic acid
Biosynthesis and genetic data: genetic data
Comments, role: Smith degraded polysaccharide.
Related record ID(s): 30652, 30832, 30834, 30835
NCBI Taxonomy refs (TaxIDs): 2233553Reference(s) to other database(s): GTC:G28921RY
Show glycosyltransferases
NMR conditions: in D2O at 303 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
3,3,3 bDManp 98.2 71.4 73.0 78.3 76.0 61.7
3,3 aDManp 103.2 68.4 78.4 66.4 74.3 62.1
3 aLFucp 102.5 68.6 78.1 72.6 68.3 16.4
2 Ac 176.1 23.4
bDGalpN 102.8 52.9 79.4 69.1 76.4 62.2
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
3,3,3 bDManp 4.85 4.11 3.80 3.75 3.47 3.67-3.85
3,3 aDManp 5.12 4.24 4.14 3.79 3.78 3.78-3.88
3 aLFucp 5.01 3.82 4.01 3.95 4.13 1.18
2 Ac - 2.05
bDGalpN 4.56 4.06 3.82 3.98 3.77 3.75-3.75
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
3,3,3 bDManp 98.2/4.85 71.4/4.11 73.0/3.80 78.3/3.75 76.0/3.47 61.7/3.67-3.85
3,3 aDManp 103.2/5.12 68.4/4.24 78.4/4.14 66.4/3.79 74.3/3.78 62.1/3.78-3.88
3 aLFucp 102.5/5.01 68.6/3.82 78.1/4.01 72.6/3.95 68.3/4.13 16.4/1.18
2 Ac 23.4/2.05
bDGalpN 102.8/4.56 52.9/4.06 79.4/3.82 69.1/3.98 76.4/3.77 62.2/3.75-3.75
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 3,3,3 | bDManp | 4.85 | 4.11 | 3.80 | 3.75 | 3.47 | 3.67
3.85 |
| 3,3 | aDManp | 5.12 | 4.24 | 4.14 | 3.79 | 3.78 | 3.78
3.88 |
| 3 | aLFucp | 5.01 | 3.82 | 4.01 | 3.95 | 4.13 | 1.18 |
| 2 | Ac |
| 2.05 | |
| | bDGalpN | 4.56 | 4.06 | 3.82 | 3.98 | 3.77 | 3.75
3.75 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 3,3,3 | bDManp | 98.2 | 71.4 | 73.0 | 78.3 | 76.0 | 61.7 |
| 3,3 | aDManp | 103.2 | 68.4 | 78.4 | 66.4 | 74.3 | 62.1 |
| 3 | aLFucp | 102.5 | 68.6 | 78.1 | 72.6 | 68.3 | 16.4 |
| 2 | Ac | 176.1 | 23.4 | |
| | bDGalpN | 102.8 | 52.9 | 79.4 | 69.1 | 76.4 | 62.2 |
|
There is only one chemically distinct structure:
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Found 1 record.
Displayed record 1
