Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Host organism: Homo sapiens
Associated disease: infection due to Escherichia coli [ICD11:
XN6P4 
]
The structure was elucidated in this paperNCBI PubMed ID: 25898391Publication DOI: 10.1016/j.carres.2015.03.014Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: perepel
ioc.ac.ru (A. V. Perepelov)
Institutions: Department of Organic Chemistry, Arrhenius Laboratory, Stockholm University, Stockholm, Sweden, N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, TEDA School of Biological Sciences and Biotechnology, Nankai University, TEDA, Tianjin, China, Department of Laboratory Medicine, Division of Clinical Microbiology, Karolinska Institute, Karolinska University Hospital, Stockholm, Sweden
The O-polysaccharides (O-antigens) were isolated by mild acid degradation of the lipopolysaccharide (LPS) of Escherichia coli O46 and O134. The structures of their linear tetrasaccharide repeating units were established by sugar analysis along with 1D and 2D (1)H and (13)C NMR spectroscopy: [Formula: see text], where D-aThr indicates D-allothreonine and R indicates O-acetyl substitution (~ 70% on aThr and ~ 15% on GalNAc) in E. coli O46 whereas the O-acetylation is absent in E. coli O134. Functions of genes in the essentially identical O-antigen gene clusters of E. coli O46 and O134 were tentatively assigned by a comparison with sequences in available databases and found to be in agreement with the O-polysaccharide structures established.
O-antigen, Escherichia coli, O-polysaccharide, bacterial polysaccharide structure, O-antigen gene cluster, threonine
Structure type: polymer chemical repeating unit
Location inside paper: abstract, p.22, chart 1, E. coli O46
The structure in this paper was incorrect:
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_115136,IEDB_130648,IEDB_136044,IEDB_137472,IEDB_137473,IEDB_140630,IEDB_141794,IEDB_142488,IEDB_146664,IEDB_190606,IEDB_423153,IEDB_983931,SB_165,SB_166,SB_187,SB_192,SB_195,SB_7,SB_88
Methods: 13C NMR, 1H NMR, NMR-2D, PCR, sugar analysis, DNA techniques, GLC, mild acid hydrolysis, de-O-acetylation, NMR-1D, GPC, function analysis of gene clusters
Biosynthesis and genetic data: genetic data
Comments, role: The OPS of E. coli O134 differs from the OPS of E. coli O46 only in the lack of O-acetylation. Title, abstract, and structure were corrcted according to Corrigendum in Carb. Res. 436 (2016) 54 : The authors regret about a mistake in the structures of the O-antigens of Escherichia coli O46 and O134. The amino acid present in these O-antigens is L-threonine, and not D-allothreonine as reported in the original version of the manuscript.
Related record ID(s): 30656
NCBI Taxonomy refs (TaxIDs): 562
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
report error