Shashkov AS, Guo X, Perepelov AV, Weintraub A, Liu B, Widmalm G, Knirel YA Structure elucidation and biosynthesis gene cluster organization of the O-antigen of Escherichia coli O170 Carbohydrate Research417 (2015)
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The structure was elucidated in this paper NCBI PubMed ID:26382081 Publication DOI:10.1016/j.carres.2015.08.013 Journal NLM ID:0043535 Publisher: Elsevier Correspondence: perepelioc.ac.ru (A.V. Perepelov) Institutions: Department of Organic Chemistry, Arrhenius Laboratory, Stockholm University, Stockholm, Sweden, N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, TEDA School of Biological Sciences and Biotechnology, Nankai University, TEDA, Tianjin, China, Department of Laboratory Medicine, Division of Clinical Microbiology, Karolinska Institute, Karolinska University Hospital, Stockholm, Sweden
Enterotoxigenic Escherichia coli are causative agents of diarrhea in humans as well as animals, and E.coli O170 belongs to this virotype. Upon mild acid degradation of the lipopolysaccharide of E.coli O170, the branched O-polysaccharide chain was partially cleaved at ?-d-glactofuranosidic linkages to give multiple products, including a linear tetrasaccharide and oligomers thereof. Studies of the acid degradation products and O-deacylated lipopolysaccharide by 1D and 2D 1H and 13C NMR spectroscopy enabled elucidation of the following O-polysaccharide structure: Functions of genes in the O-antigen biosynthesis gene cluster were tentatively assigned and found to be in agreement with the O-polysaccharide structure.
Methods: 13C NMR, 1H NMR, NMR-2D, de-O-acylation, sugar analysis, acid hydrolysis, GLC, mild acid hydrolysis, NMR-1D, GPC Comments, role: Fraction III, OS 2 was obtained after full acid hydrolysis of the OPS.
Related record ID(s): 30672, 30862, 30864 NCBI Taxonomy refs (TaxIDs):562 Reference(s) to other database(s): GTC:G78157OI Show glycosyltransferases
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ioc.ac.ru (A.V. Perepelov)