Taxonomic group: bacteria / Proteobacteria (Phylum: Proteobacteria)
Host organism: Homo sapiens
Associated disease: infection due to Acinetobacter baumannii [ICD11: XN8LS ]
 
The structure was elucidated in this paper
NCBI PubMed ID: 25665782
Publication DOI: 10.1016/j.carres.2014.11.013
Journal NLM ID: 0043535
Publisher: Elsevier
Correspondence: Y.A. Knirel <yknirelgmail.com>
Institutions: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, State Research Center for Applied Microbiology and Biotechnology, Obolensk, Moscow Region, Russia, Moscow Institute of Physics and Technology, Dolgoprudny, Moscow Region, Russia, M.M. Shemyakin & Y.A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia, Research Institute of Physical-Chemical Medicine, Moscow, Russia, Institute of Fundamental Medicine and Biology, Kazan (Volga Region) Federal University, Kazan, Russia

Acinetobacter baumannii 1053 is the type strain for the maintenance of specific bacteriophage AP22, which infects a fairly broad range of A. baumannii strains circulating in Russian clinics and hospitals. A capsular polysaccharide (CPS) was isolated from cells of strain 1053 and studied by sugar analysis along with 1D and 2D (1)H and (13)C NMR spectroscopy. The following structure of the linear trisaccharide repeating unit was established: →4)-β-D-ManpNAcA-(1→4)-β-D-ManpNAcA-(1→3)-α-D-FucpNAc-(1→ where ManNAcA and FucNAc indicate 2-acetamido-2-deoxymannuronic acid and 2-acetamido-2,6-dideoxygalactose, respectively. A polysaccharide having the same repeating unit but a shorter chain was isolated by the phenol-water extraction of bacterial cells. Sequencing of the CPS biosynthesis gene locus showed that A. baumannii 1053 belongs to a new group designated KL91. The gene functions assigned putatively by a comparison with available databases were in agreement with the CPS structure established.

Acinetobacter baumannii, 2-acetamido-2, 6-dideoxy-D-galactose, capsular polysaccharide structure, 2-acetamido-2-deoxy-D-mannuronic acid, Polysaccharide biosynthesis gene locus

Structure type: fragment of a bigger structure
Location inside paper: p.80, chart 1, PS
Trivial name: polysaccharide (PS)
 
Methods: 13C NMR, 1H NMR, NMR-2D, PCR, DNA sequencing, sugar analysis, acid hydrolysis, GLC, mild acid hydrolysis, NMR-1D, GPC, extraction, bioinformatic analysis
Biosynthesis and genetic data: genetic data
Comments, role: Fragment with the terminal non-reducing residue bDManpNAcA in the PS; PS was isolated from cells of A. baumannii 1053 by phenol-water extraction followed by cleavage of the accompanying lipopolysaccharide by mild acid hydrolysis. PS consists on the average of four trisaccharide repeats.
 
Related record ID(s): 30675
NCBI Taxonomy refs (TaxIDs): 470
Reference(s) to other database(s): GTC:G93871SB
Show glycosyltransferases
 
NMR conditions: in D2O at 293 K       [as TSV]

1H NMR data: Linkage Residue H1 H2 H3 H4 H5 H6 3,4,2 Ac 1.99 2.08   3,4 bDManpNA 4.75 4.44 3.79 3.59 3.80 3,2 Ac 1.99 2.08   3 bDManpNA 4.89 4.43 3.87 3.76 3.83 2 Ac 1.99 2.08     aDFucpN 5.20 4.20 3.93 3.96 4.02 1.17

13C NMR data: Linkage Residue C1 C2 C3 C4 C5 C6 3,4,2      Ac 175.5 176.6 23.2 23.3   3,4      bDManpNA 100.1 54.4 74.4 70.1 77.6 175.5 3,2      Ac 175.5 176.6 23.2 23.3   3      bDManpNA 100.9 53.9 71.5 78.9 76.4 175.2 2      Ac 175.5 176.6 23.2 23.3          aDFucpN 98.9 48.8 79.1 71.3 68.1 16.6

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