Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Host organism: Homo sapiens
Associated disease: urinary tract infections (UTI) [ICD11:
GC08 
];
infection due to Morganella [ICD11:
XN8G6 ]
The structure was elucidated in this paperNCBI PubMed ID: 25498024Publication DOI: 10.1016/j.carres.2014.10.032Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: Evguenii.Vinogradov
nrc-cnrc.gc.ca
Institutions: Human Health Therapeutics Portfolio, National Research Council of Canada, 100 Sussex Drive, Ottawa, ON K1A 0R6, Canada, Public Health Agency of Canada, Toronto, ON, Canada
The core region of the lipopolysaccharide of Morganella morganii serotype O:1ab was obtained by hydrolysis of the LPS and studied by 2D NMR, ESI MS, and chemical methods. Its structure was highly homologous to those from the two major members of the same Proteeae tribe, Proteus mirabilis and Providencia alcalifaciens, and analysis of the M. morganii genome disclosed that the loci for its outer core, lipid A and Ara4N moieties are similarly conserved.
LPS, core, Morganella morganii, carbohydrate gene loci
Structure type: oligomer
Location inside paper: p.233, fig.2, oligosaccharide 2
Compound class: core oligosaccharide
Contained glycoepitopes: IEDB_136906,IEDB_137472,IEDB_140529,IEDB_141794,IEDB_142488,IEDB_146664,IEDB_151528,IEDB_167069,IEDB_190606,IEDB_983931,SB_192,SB_7
Methods: 13C NMR, 1H NMR, methylation, NMR-2D, DNA sequencing, GC-MS, de-O-acylation, sugar analysis, dephosphorylation, 31P NMR, ESI-MS, acid hydrolysis, HPAEC, GC, deamination, GPC, UV
Comments, role: Oligosaccharide 2 - product of deamination of the core oligosaccharide.
Related record ID(s): 30687, 30887
NCBI Taxonomy refs (TaxIDs): 582Reference(s) to other database(s): GTC:G21762EV
Show glycosyltransferases
NMR conditions: in D2O at 298 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
3,6 aDGalp 99.4 69.5 70.7 70.4 72.2 62.3
3 bDGlcp 104.0 74.0 76.8 70.8 75.5 67.1
6 %xXP?
xD2,5anhTala 90.9 82.8 82.9 73.0 82.3 61.2
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
3,6 aDGalp 5.00 3.84 3.87 3.99 3.94 3.75-3.75
3 bDGlcp 4.62 3.39 3.51 3.51 3.67 3.78-3.96
6 %xXP?
xD2,5anhTala 5.08 3.93 4.41 4.39 4.13 3.78-3.83
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
3,6 aDGalp 99.4/5.00 69.5/3.84 70.7/3.87 70.4/3.99 72.2/3.94 62.3/3.75-3.75
3 bDGlcp 104.0/4.62 74.0/3.39 76.8/3.51 70.8/3.51 75.5/3.67 67.1/3.78-3.96
6 %xXP?
xD2,5anhTala 90.9/5.08 82.8/3.93 82.9/4.41 73.0/4.39 82.3/4.13 61.2/3.78-3.83
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 3,6 | aDGalp | 5.00 | 3.84 | 3.87 | 3.99 | 3.94 | 3.75
3.75 |
| 3 | bDGlcp | 4.62 | 3.39 | 3.51 | 3.51 | 3.67 | 3.78
3.96 |
| 6 | %xXP? | |
| | xD2,5anhTala | 5.08 | 3.93 | 4.41 | 4.39 | 4.13 | 3.78
3.83 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 3,6 | aDGalp | 99.4 | 69.5 | 70.7 | 70.4 | 72.2 | 62.3 |
| 3 | bDGlcp | 104.0 | 74.0 | 76.8 | 70.8 | 75.5 | 67.1 |
| 6 | %xXP? | |
| | xD2,5anhTala | 90.9 | 82.8 | 82.9 | 73.0 | 82.3 | 61.2 |
|
There is only one chemically distinct structure:
Found 
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