Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Host organism: Homo sapiens
Associated disease: neonatal necrotising enterocolitis [ICD11:
KB88 
, Life stage: neonatal];
bacteremia [ICD11:
MA15.0 ];
septicemia [ICD11:
MA15.Y ];
meningitis [ICD11:
1D01 ]
The structure was elucidated in this paperPublication DOI: 10.1007/s11172-015-0999-yJournal NLM ID: 100912060Publisher: New York: Consultants Bureau
Correspondence: yknirel
gmail.com (Y.A. Knirel)
Institutions: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, TEDA Institute of Biological Sciences and Biotechnology, Nankai University, Tianjin Economic-Technological Development Area, Tianjin, 300457, People´s Republic of China, Key Laboratory of Molecular Microbiology and Technology of the Ministry of Education, College of Life Sciences, Nankai University, Tianjin, 300071, People’s Republic of China
Partially O-acetylated fructan was isolated by phenol—water extraction of cells of Cronobacter dublinensis G2732 followed by anion-exchange chromatography on DEAE-Toyopearl 650M. Structure of the isolated fructan was established by 2D 1H and 13C NMR spectroscopy. The same fructan but lacking O-acetyl groups was isolated from two other C. dublinensis strains, G3947 and G4061. Genes for the fructan biosynthesis were not found at the typical Cronobacter O-antigen gene cluster located between the conserved genes galF and gnd and are evidently located elsewhere on the chromosome.
Lipopolysaccharide, bacterial polysaccharide structure, fructan, Cronobacter dublinensis
Structure type: polymer chemical repeating unit
Location inside paper: p.1194, table 1
Compound class: O-polysaccharide
Methods: 13C NMR, 1H NMR, NMR-2D, sugar analysis, mild acid hydrolysis, GPC
Comments, role: OPS from C. dublinensis: G3947, G4061 and de-O-acetylated OPS from C. dublinensis G2732.
Related record ID(s): 30583
NCBI Taxonomy refs (TaxIDs): 413497Reference(s) to other database(s): GTC:G38641ZB
Show glycosyltransferases
NMR conditions: in D2O at 303 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
1 aDFruf 60.4 109.2 82.3 78.4 84.9 62.6
bDFruf 61.1 104.2 78.4 75.8 82.5 63.3
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
1 aDFruf 3.77-3.98 - 4.17 4.02 4.06 3.73-3.85
bDFruf 3.82-3.83 - 4.31 4.17 3.88 3.73-3.87
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
1 aDFruf 60.4/3.77-3.98 82.3/4.17 78.4/4.02 84.9/4.06 62.6/3.73-3.85
bDFruf 61.1/3.82-3.83 78.4/4.31 75.8/4.17 82.5/3.88 63.3/3.73-3.87
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 1 | aDFruf | 3.77
3.98 |
| 4.17 | 4.02 | 4.06 | 3.73
3.85 |
| | bDFruf | 3.82
3.83 |
| 4.31 | 4.17 | 3.88 | 3.73
3.87 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 1 | aDFruf | 60.4 | 109.2 | 82.3 | 78.4 | 84.9 | 62.6 |
| | bDFruf | 61.1 | 104.2 | 78.4 | 75.8 | 82.5 | 63.3 |
|
There is only one chemically distinct structure:
Found 
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(previously named: Enterobacter sakazakii G2732)