Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Host organism: Rattus rattus; Xenopsylla cheopis
Associated disease: endemic typhus (rickettsiosis) [ICD11:
1C30.2 
, ICD11:
XN2AR ];
infection due to Rickettsia typhi [ICD11:
XN2AR ]
The structure was elucidated in this paperNCBI PubMed ID: 26435145Journal NLM ID: 0370401Publisher: Bratislava, Slovak Republic: AEPress, Ltd
Correspondence: virutoma
savba.sk
Institutions: Department of Rickettsiology, Institute of Virology, Slovak Academy of Sciences, Dúbravská cesta 9,84505 Bratislava, Slovak Republic, Medical Biochemistry, Gothenburg University, Box 440, 405 30 Gothenburg, Sweden
Elucidation of the O-specific polysaccharide chain of lipopolysaccharide (LPS) from Rickettsia typhi, the etiological agent of endemic typhus, is described. Structural information was established by a combination of monosaccharide and methylation analyses of the O-chain, and by mass (MS) and nuclear magnetic resonance (NMR) spectrometries of oligosaccharides arised through its hydrofluoric (HF) acid degradation. Based on the combined data from these experiments, two major polymer populations of the O-specific chain have been determined with the following structural features: α-L-QuiNAc-(1→4)-[α-D-Glc-(1→3)-α-L-QuiNAc-(1→4)]n-α-D-Glc- (1→4)-α-D-Glc→, α-D-Glc-(1→3)-α-L-QuiNAc-(1→4)-[α-D-Glc-(1→3)-α-L-QuiNAc-(1→4)]n-α-D-Glc→. The linear backbone is most probably flanked with short side chains of D-GlcNAc-(1→3)-α-L-QuiNAc-(1→3)-D-GlcNAc→ that are attached to it via L-QuiNAc as a branching point. It is suggested that a dimer α-L-QuiNAc-(1→3)-α-D-GlcNAc may represent a common epitope in the O-antigens of Proteus vulgaris OX19 and R. typhi responsible for the observed serological cross-reactivity.
Lipopolysaccharide, structure, O-antigens, chemical composition, Rickettsia typhi
Structure type: fragment of a bigger structure
Location inside paper: abstract, p.232
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_142488,IEDB_144998,IEDB_146664,IEDB_983931,SB_192
Methods: 13C NMR, 1H NMR, methylation, NMR-2D, GC-MS, sugar analysis, ESI-MS, mild acid hydrolysis, HPLC, HF treatment
Comments, role: Variant of structure (see ID: 30973), two major polymer fraction of the O-antigen was obtained by treatment LPS with mild acid hydrolysis and HF. Disaccharide fragment [aDGlcp(1-3)[Ac(1-2)]aLQuipN(1-4)] in the O-antigen may be longer, n=2-5.
Related record ID(s): 30726, 30973, 30975
NCBI Taxonomy refs (TaxIDs): 785Reference(s) to other database(s): GTC:G76668KA
Show glycosyltransferases
There is only one chemically distinct structure:
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