Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Escherichia coli [ICD11:
XN6P4 
]
The structure was elucidated in this paperNCBI PubMed ID: 11298740Journal NLM ID: 0107600Publisher: Oxford, UK: Blackwell Science Ltd. on behalf of the Federation of European Biochemical Societies
Correspondence: G. Widmalm <gw
organ.su.se>
Institutions: Department of Organic Chemistry, Arrhenius Laboratory, Stockholm University, Sweden, Karolinska Institute, Department of Microbiology, Pathology and Immunology, Division of Clinical Bacteriology, Huddinge University Hospital, Sweden
The structure of the O-antigen polysaccharide from Escherichia coli O172 has been determined. In combination with sugar analysis, NMR spectroscopy shows that the polysaccharide is composed of pentasaccharide repeating units. Sequential information was obtained by mass spectrometry and two-dimensional NMR techniques. An O-acetyl group was present as 0.7 equivalent per repeating unit. Treatment of the O-deacetylated polysaccharide with aqueous 48% hydrofluoric acid rendered cleavage of the phosphodiester in the backbone of the polymer and the pentasaccharide isolated after gel permeation chromatography was structurally characterized. Subsequent NMR experiments on polymeric materials revealed the structure of the repeating unit of the O-polysaccharide from E. coli O172 as: →P-4)-α-D-Glcp-(1→3)-α-L-FucpNAc-(1→3)-α-D-GlcpNAc-(1→3)-α-L-FucpNAc-(1→4)-α-D-Glcp6Ac-(1→.
structural, polysaccharide, O-antigen, analysis, O antigen, Escherichia, Escherichia coli, structural analysis
Structure type: suggested polymer biological repeating unit
Location inside paper: abstract
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_137340,IEDB_141807,IEDB_142488,IEDB_144998,IEDB_145002,IEDB_146664,IEDB_151531,IEDB_983931,SB_192
Methods: NMR-2D, NMR, HF solvolysis, sugar analysis, de-O-acetylation
Comments, role: chemical repeat frame is different in the paper
Related record ID(s): 3208, 8981, 20708, 21747, 21772
NCBI Taxonomy refs (TaxIDs): 562Reference(s) to other database(s): GTC:G58779ZT, GlycomeDB:
28138
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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