Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Host organism: Homo sapiens
Associated disease: nosocomial infections [ICD11:
XB25 
];
infection due to Pseudomonas aeruginosa [ICD11:
XN5L6 ]
The structure was elucidated in this paperNCBI PubMed ID: 15170746Publication DOI: 10.1002/jms.611Journal NLM ID: 9504818Publisher: Chichester, UK: Wiley
Correspondence: gilles.bedoux
uni-ubs.fr
Institutions: Laboratoire de Biologie et Chimie Moléculaires, Université de Bretagne Sud, Lorient, France, Laborgruppe Immunchemie, Forschungszentrum Borstel, Zentrum für Medizin und Biowissenschaften, Borstel, Germany
The lipid A components of the Pseudomonas aeruginosa strains PAO1 (wild-type) and derived mutants PAO1 algC::tet and PAO1 PDO100 were isolated after mild acetic acid hydrolysis of LPS. Their structural heterogeneities were characterized using electrospray ionization (ESI) ion-trap mass spectrometry (MS) with direct infusion in the negative ion mode without prior derivatization. The ESI-mass spectra revealed monophosphorylated molecules corresponding to known tetra-, penta- and hexaacylated structures of P. aeruginosa lipid A. The MS/MS fragmentation patterns allowed the location of fatty acyl chains on the disaccharide backbone of lipid A. In addition, a hexaacylated lipid A containing a hexadecanoyl chain was detected for the first time in strain P. aeruginosa PAO1. With multiple stages of fragmentation (MS(n)), the position of this hexadecanoyl chain O-linked to the decanoyl chain at the C-3(') position of the glucosamine backbone was determined. This sensitive method is suitable to reveal lipid A heterogeneity, i.e. the nature, number and distribution of acyl chains, without prior lipopolysaccharide purification. The lipid A from mutant strains were also characterized and significant differences were shown in the abundance of monophosphorylated lipid A components between the wild-type and the mutant strains.
Lipopolysaccharide, Pseudomonas aeruginosa, lipid A, electrospray ionization mass spectrometry, ion-trap analyzer
Structure type: oligomer ; 1365.9
Location inside paper: p.510, fig.2A
Compound class: lipid A
Contained glycoepitopes: IEDB_141807,IEDB_151531,IEDB_534864
Methods: SDS-PAGE, ESI-MS, GLC, mild acid hydrolysis, MS/MS, composition analysis, HPLC
Comments, role: major lipid A obtained from wild-type P. aeruginosa serotype O5 strain PAO1
Related record ID(s): 9552, 31321
NCBI Taxonomy refs (TaxIDs): 208964
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There is only one chemically distinct structure:
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