Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Host organism: Homo sapiens
Associated disease: hemorrhagic colitis (HC) [ICD11:
1A40.0 
];
hemolytic-uremic syndrome (HUS) [ICD11:
3A21.2 ];
infection due to Escherichia coli [ICD11:
XN6P4 ]
The structure was elucidated in this paperNCBI PubMed ID: 15170747Publication DOI: 10.1002/jms.614Journal NLM ID: 9504818Publisher: Chichester, UK: Wiley
Correspondence: byungkim
snu.ac.kr
Institutions: School of Chemical Engineering and Institute of Molecular Biology and Genetics, Seoul National University, Kwanak-Gu, Seoul 151-742, Korea
Rapid separation and structural identification of lipid A from Escherichia coli were performed using thin-layer chromatography (TLC) and mass spectrometry (MS). After the resolved spot of the lipid A had been scraped from TLC plate, the sample was re-extracted from the removed powder with chloroform-methanol (2 : 1, v/v) and analyzed by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) and electrospray ionization (ESI) ion-trap MS. For detailed structural characterization, multiple-stage mass analysis (MS(4)) of the major species in ESI-MS/MS provided important information about the series of fragment ions. The dominant fragment ions in each MS stage were produced from the loss of fatty acyl groups mainly driven by charge-remote processes, and this information about the fragment ions can be used to deduce the composition or the position of the fatty acid substituent in the lipid A. In contrast, MALDI-TOFMS indicated that fragmentation resulted from charge-driven processes. Molecular mass profiling and fragmentation analysis provides essential information for clarifying the detailed structure of the lipid A from E. coli O157:H7:K(-).
Escherichia coli O157, lipid A, structural analysis, thin-layer chromatography, multiple stage mass analysis
Structure type: oligomer ; 1359.5
Location inside paper: p.517, fig.3
Compound class: lipid A
Contained glycoepitopes: IEDB_135394,IEDB_141807,IEDB_151531,IEDB_534864
Methods: ESI-MS, mild acid hydrolysis, MALDI-TOF MS, PD-MS, CID-MS/MS
Related record ID(s): 9551, 31322, 31324
NCBI Taxonomy refs (TaxIDs): 83334
Show glycosyltransferases
There is only one chemically distinct structure:
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