Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Host organism: Homo sapiens
Associated disease: meningitis [ICD11:
1D01 
];
septicemia [ICD11:
MA15.Y ];
infection due to Neisseria meningitidis [ICD11:
XN1DV ]
The structure was elucidated in this paperNCBI PubMed ID: 11948150Publication DOI: 10.1128/JB.184.9.2379-2388.2002Journal NLM ID: 2985120RPublisher: American Society for Microbiology
Correspondence: dstep01
emory.edu
Institutions: Division of Infectious Diseases, Department of Medicine, Emory University School of Medicine, Atlanta, GA 30303, USA
Lipopolysaccharide, lipooligosaccharide (LOS), or endotoxin is important in bacterial survival and the pathogenesis of gram-negative bacteria. A necessary step in endotoxin biosynthesis is 3-deoxy-D-manno- octulosonic acid (Kdo) glycosylation of lipid A, catalyzed by the Kdo transferase KdtA (WaaA). In enteric gram-negative bacteria, this step is essential for survival. A nonpolar kdtA::aphA-3 mutation was created in Neisseria meningitidis via allelic exchange, and the mutant was viable. Detailed structural analysis demonstrated that the endotoxin of the kdtA::aphA-3 mutant was composed of fully acylated lipid A with variable phosphorylation but without Kdo glycosylation. In contrast to what happens in other gram-negative bacteria, tetra-acylated lipid IV(A) did not accumulate. The LOS structure of the kdtA::aphA-3 mutant was restored to the wild-type structure by complementation with kdtA from N. meningitidis or Escherichia coli. The expression of a fully acylated, unglycosylated lipid A indicates that lipid A biosynthesis in N. meningitidis can proceed without the addition of Kdo and that KdtA is not essential for survival of the meningococcus.
LPS, structure, Lipooligosaccharide, Neisseria meningitidis, Escherichia coli, lipid A, Gram-negative bacteria, kdtA
Structure type: oligomer ; 1757
Location inside paper: p.2385, fig.6
Compound class: lipid A
Contained glycoepitopes: IEDB_120354,IEDB_123890,IEDB_141807,IEDB_151531,IEDB_534864
Methods: methylation, PCR, GC-MS, HF solvolysis, DNA techniques, mild acid hydrolysis, GC, Western blotting, MS/MS, MALDI-TOF MS, composition analysis, genetic methods, tricine-SDS-PAGE, electron microscopy
Comments, role: kdtA mutant
Related record ID(s): 5246, 31374, 31375
NCBI Taxonomy refs (TaxIDs): 1172206
Show glycosyltransferases
There is only one chemically distinct structure:
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