Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Bordetella pertussis [ICD11:
XN23B 
]
NCBI PubMed ID: 15930524Journal NLM ID: 0376606Publisher: ASBMB
Correspondence: martine.caroff
bbmpc.u-psud.fr
Institutions: Equipe Endotoxines, Unité Mixte de Recherche 8619 du Centre National de la Recherche Scientifique, Institut de Biochimie, Biophysique Moléculaire et Cellulaire, Université de Paris-Sud, Orsay, France, Department of Physical Chemistry, University of Bucharest, Bucharest, Romania
Endotoxins (lipopolysaccharides) are the main components of Gram-negative bacterial outer membranes. A quick and simple way to isolate their lipid region (lipid A) directly from whole bacterial cells was devised. This method using hot ammonium-isobutyrate solvent was applied to small quantities of cells and proved to be indispensable when a rapid characterization of lipid A structure by mass spectrometry was required. Biological activities of endotoxins are directly related to the lipid A structures, which vary greatly with cell growth conditions. This method is suitable for rough- and smooth-type bacteria and very efficient for screening variations in lipid A structures. Data are acquired in a few hours and avoid the use of phenol in extraction.
Lipopolysaccharide, hydrolysis, endotoxin
Structure type: oligomer ; 1559
Location inside paper: p.1774, fig.3B
Compound class: lipid A
Contained glycoepitopes: IEDB_135394,IEDB_135515,IEDB_141807,IEDB_151531,IEDB_176772
Methods: TLC, acid hydrolysis, MALDI-MS, detergent-promoted hydrolysis
Comments, role: methods;
Related record ID(s): 10031, 31496
NCBI Taxonomy refs (TaxIDs): 520
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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