Taxonomic group: bacteria / Firmicutes
(Phylum: Firmicutes)
Associated disease: infection due to Streptococcus pneumoniae [ICD11:
XN3PW 
]
Journal NLM ID: 2985121RPublisher: Baltimore, MD: American Society for Biochemistry and Molecular Biology
Correspondence: e.garcia
cib.csic.es
Institutions: Centro de Investigaciones Biologicas, CSIC, Velazquez 144, 28006 Madrid, Spain
The type 37 capsule of Streptococcus pneumoniae is a homopolysaccharide built up from repeating units of [b-D-Glcp-(1→2)]-b-D-Glcp-(1→3). The elements governing the expression of the tts gene, coding for the glucosyltransferase involved in the synthesis of the type 37 pneumococcal capsular polysaccharide, have been studied. Primer extension analysis and functional tests demonstrated the presence of four new transcriptional start points upstream of the previously reported tts promoter (ttsp). Most interesting, three of these transcriptional start points are located in a RUP element thought to be involved in recombinational events (Oggioni, M. R., and Claverys, J. P. (1999) Microbiology 145, 2647-2653). Transformation experiments using either a recombinant plasmid containing the whole transcriptional unit of tts or chromosomal DNA from a type 37 pneumococcus showed that tts is the only gene required to drive the biosynthesis of a type 37 capsule in S. pneumoniae and other Gram-positive bacteria, namely Streptococcus oralis, Streptococcus gordonii, and Bacillus subtilis. The Tts synthase was overproduced in S. pneumoniae and purified as a membrane-associated enzyme. These membrane preparations used UDP-Glc as substrate to catalyze the synthesis of a high molecular weight polysaccharide immunologically identical to the type 37 capsule. In addition, UDP-Gal was also a substrate to produce type 37 polysaccharide since a strong UDP-Glc-4*-epimerase activity is associated to the membrane fraction of S. pneumoniae. These results indicated that Tts has a dual biochemical activity that leads to the synthesis of the branched type 37 polysaccharide.
synthesis, branched, capsular, polysaccharide, Streptococcus, Streptococcus pneumoniae, capsular polysaccharide, type, species, Gram-positive, Pneumococcus
Structure type: polymer chemical repeating unit
Location inside paper: abstract
Trivial name: β-1,3/1,2-glucan
Compound class: CPS, EPS, glucan
Contained glycoepitopes: IEDB_1397514,IEDB_140628,IEDB_142488,IEDB_146664,IEDB_153543,IEDB_158555,IEDB_161166,IEDB_2278476,IEDB_2278477,IEDB_558869,IEDB_857743,IEDB_983931,SB_192
Biosynthesis and genetic data: genetic data, biosynthetic data
Related record ID(s): 10089, 10112
NCBI Taxonomy refs (TaxIDs): 1313Reference(s) to other database(s): GTC:G32470ZC, GlycomeDB:
25916
Show glycosyltransferases
There is only one chemically distinct structure:
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