Taxonomic group: bacteria / Chlamydiae
(Phylum: Chlamydiae)
Associated disease: infection due to Chlamydia pneumoniae [ICD11:
XN9EE 
]
NCBI PubMed ID: 8748024Journal NLM ID: 8712028Publisher: Blackwell Publishing
Institutions: Division of biochemical Microbiology, Forschungsinstitut Borstel, Borstel, Germany.
The gene kdtA of Chlamydia pneumoniae strain TW-183, encoding the enzyme 3-deoxy-α-D-manno-octulosonic acid (Kdo) transferase of lipopolysaccharide biosynthesis, was cloned and sequenced. A single open reading frame of 1314 bp was identified, the deduced amino acid sequence of which revealed 69% similarity and 43% identity with KdtA of Chlamydia trachomatis and Chlamydia psittaci. The gene was expressed in the Gram-positive host Corynebacterium glutamicum and the primary gene product was characterized as a multifunctional glycosyltransferase. Cell-free extracts generated in vitro the genus-specific epitope of Chlamydia composed of the trisaccharide αKdo(2-8)αKdo(2-4)αKdo. The results show that a single polypeptide affords three different glycosidic bonds, which is in contradiction to the dogma of glycobiology: 'one enzyme - one glycosidic bond'.
Lipopolysaccharide, LPS, biosynthetic, functional, gene, Sequence Analysis, strain, characterization, analysis, molecular, acid, cloning, transferase, Chlamydia, sequence
Structure type: oligomer
Location inside paper: abstract
Aglycon: lipid A
Contained glycoepitopes: IEDB_130650,IEDB_130658
Methods: biosynthetic methods
Biological activity: genus-specific epitope
Enzymes that release or process the structure: 3-deoxy-α-D-manno-octulosonic acid transferase
Biosynthesis and genetic data: biosynthetic data, genetic data
Comments, role: genus-specific epitope
NCBI Taxonomy refs (TaxIDs): 182082Reference(s) to other database(s): GTC:G00047ZG, GlycomeDB:
25919
Show glycosyltransferases
There is only one chemically distinct structure:
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