Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Escherichia coli [ICD11:
XN6P4 
]
The structure was elucidated in this paperNCBI PubMed ID: 8839182Journal NLM ID: 0043535Publisher: Elsevier
Institutions: Department of Organic Chemistry, Arrhenius Laboratory, Stockholm University, Stockholm, Sweden, Karolinska Institute, Department of Immunology, Microbiology, Pathology and Infectious Diseases, Division of Clinical Bacteriology, Huddinge University Hospital, Huddinge, Sweden
The structure of the O-specific side chain of the E. coli O26 lipopolysaccharide has been investigated. Based on sugar and methylation analyses, and 2D NMR spectroscopy employing HMBC experiments, it is concluded that the polysaccharide is composed of trisaccharide repeating units having the following structure. →3)-α-L-Rhap-(1→4)-α-L-FucpNAc-(1→3)-β-D-GlcpNAc-(1→.
Lipopolysaccharide, LPS, structural, polysaccharide, O-antigen, O antigen, Escherichia, Escherichia coli, structural studies, enterohemorrhagic
Structure type: polymer chemical repeating unit
Location inside paper: abstract
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_135813,IEDB_136105,IEDB_137340,IEDB_141807,IEDB_151531,IEDB_225177,IEDB_885823
Methods: methylation, NMR-2D, NMR, sugar analysis
Related record ID(s): 10023, 20653, 23615
NCBI Taxonomy refs (TaxIDs): 404399Reference(s) to other database(s): GTC:G67201SZ, GlycomeDB:
25922
Show glycosyltransferases
NMR conditions: in D2O at 353 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
3,4 aLRhap 102.6 70.9 81.1 71.7 70.4 17.4
3,2 Ac 175.1 23.0
3 aLFucpN 98.6 50.6 68.0 82.0 68.0 16.3
2 Ac 175.0 23.0
bDGlcpN 103.5 56.5 79.6 69.4 76.4 61.5
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
3,4 aLRhap 4.83 4.28 3.90 3.49 4.03 1.22
3,2 Ac - 1.97
3 aLFucpN 5.01 4.17 3.96 3.87 4.43 1.19
2 Ac - 2.04
bDGlcpN 4.68 3.89 3.70 3.55 3.47 3.77-3.89
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
3,4 aLRhap 102.6/4.83 70.9/4.28 81.1/3.90 71.7/3.49 70.4/4.03 17.4/1.22
3,2 Ac 23.0/1.97
3 aLFucpN 98.6/5.01 50.6/4.17 68.0/3.96 82.0/3.87 68.0/4.43 16.3/1.19
2 Ac 23.0/2.04
bDGlcpN 103.5/4.68 56.5/3.89 79.6/3.70 69.4/3.55 76.4/3.47 61.5/3.77-3.89
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 3,4 | aLRhap | 4.83 | 4.28 | 3.90 | 3.49 | 4.03 | 1.22 |
| 3,2 | Ac |
| 1.97 | |
| 3 | aLFucpN | 5.01 | 4.17 | 3.96 | 3.87 | 4.43 | 1.19 |
| 2 | Ac |
| 2.04 | |
| | bDGlcpN | 4.68 | 3.89 | 3.70 | 3.55 | 3.47 | 3.77
3.89 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 3,4 | aLRhap | 102.6 | 70.9 | 81.1 | 71.7 | 70.4 | 17.4 |
| 3,2 | Ac | 175.1 | 23.0 | |
| 3 | aLFucpN | 98.6 | 50.6 | 68.0 | 82.0 | 68.0 | 16.3 |
| 2 | Ac | 175.0 | 23.0 | |
| | bDGlcpN | 103.5 | 56.5 | 79.6 | 69.4 | 76.4 | 61.5 |
|
There is only one chemically distinct structure:
Found 
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