Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Host organism: Homo sapiens
Associated disease: bacteremia [ICD11:
MA15.0 
];
septicemia [ICD11:
MA15.Y ];
respiratory diseases [ICD11:
12 ]
The structure was elucidated in this paperNCBI PubMed ID: 19706748Publication DOI: 10.1194/jlr.M001362Journal NLM ID: 0376606Publisher: ASBMB
Correspondence: czaja
iitd.pan.wroc.pl
Institutions: Department of Chemistry, Swedish University of Agricultural Sciences, Uppsala, Sweden, L. Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroclaw, Poland, Department of Biotechnology and Molecular Biology, University of Opole, Opole, Poland
Hafnia alvei, a Gram-negative bacterium, is an opportunistic pathogen associated with mixed hospital infections, bacteremia, septicemia, and respiratory diseases. The majority of clinical symptoms of diseases caused by this bacterium have lipopolysaccharide (LPS, endotoxin)-related origin. The lipid A structure affects the biological activity of endotoxins predominantly. Thus the structure of H. alvei lipid A was analyzed for the first time. The major form, asymmetrically hexa-acylated lipid A built of β-D-GlcpN4P-(1→6)-α-D-GlcpN1P substituted with (R)-14:0(3-OH) at N-2 and O-3, 14:0(3-(R)-O-12:0) at N-2', and 14:0(3-(R)-O-14:0) at O-3', was identified by ESI-MS(n) and MALDI-TOF MS. Comparative analysis performed by mass spectrometry suggested that LPSs of H. alvei 32, PCM 1192, PCM 1206 and PCM 1207 share the identified structure of lipid A. Lipopolysaccharides of H. alvei are yet another example of enterobacterial endotoxins having the E. coli-type structure of lipid A. The presence of hepta-acylated forms of H. alvei lipid A resulted from the addition of palmitate (16:0) substituting 14:0(3-OH) at N-2 of the α-GlcpN residue. All the studied strains of H. alvei have an ability to modify their lipid A structure by palmitoylation.
endotoxin, Hafnia alvei, mass spectrometry, Polish Collection of Microorganisms, palmitoylation
Structure type: oligomer ; 1796.4
Location inside paper: p.567, fig.1, p.568, fig.2
Compound class: lipid A
Contained glycoepitopes: IEDB_135394,IEDB_135515,IEDB_141807,IEDB_151531,IEDB_176772,IEDB_534864
Methods: GC-MS, de-O-acylation, dephosphorylation, ESI-MS/MS, chemical methods, MALDI-TOF MS, de-N-acylation
Related record ID(s): 25332, 32011
NCBI Taxonomy refs (TaxIDs): 569
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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