Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: pneumonia [ICD11:
CA40 
];
urinary tract infections (UTI) [ICD11:
GC08 ];
infection due to Klebsiella pneumoniae [ICD11:
XN741 ]
The structure was elucidated in this paperNCBI PubMed ID: 32224183Publication DOI: 10.1016/j.ijbiomac.2020.03.196Journal NLM ID: 7909578Publisher: Butterworth-Heinemann
Correspondence: pcescutti
units.it
Institutions: Department of Medical Biotechnologies, University of Siena, Siena, Italy, Department of Biology, University of Rome 'Tor Vergata', Rome, Italy, Department of Experimental and Clinical Medicine, University of Florence, Florence, Italy, Microbiology and Virology Unit, Florence Careggi University Hospital, Florence, Italy, Department of Life Sciences, University of Trieste, 34127 Trieste, Italy
Klebsiella pneumoniae strain KPB-1 was isolated in early 2011 from the pleural fluid of an inpatient admitted at an Italian hospital. It was characterized to produce the KPC-3 carbapenemase and to belong to sequence type 512, a derivative of sequence type 258 clade II characterized by the cps-2 gene cluster. The K-antigen of K. pneumoniae KPB-1 was purified and its structure determined by using GLC-MS of appropriate carbohydrate derivatives and 1D and 2D NMR spectroscopy of the native polysaccharide. All the collected data demonstrated the following repeating unit for the K. pneumoniae KPB-1 capsular polysaccharide: The reactions catalysed by each glycosyltransferase in the cps-2 gene cluster were assigned on the basis of structural homology with other Klebsiella K antigens.
NMR, glycosyltransferases, capsular polysaccharide structure, Klebsiella pneumoniae KPB-1
Structure type: polymer chemical repeating unit
Location inside paper: abstract, p.317, table 1, CPS KPB-1
Compound class: CPS
Contained glycoepitopes: IEDB_133754,IEDB_136044,IEDB_136105,IEDB_137472,IEDB_141794,IEDB_144825,IEDB_190606,IEDB_225177,IEDB_885823,SB_165,SB_166,SB_187,SB_195,SB_7,SB_88
Methods: 13C NMR, 1H NMR, GLC-MS, NMR-2D, sugar analysis, GLC, function analysis of gene clusters
Comments, role: published polymerization frame was shifted for conformity with other records.
Related record ID(s): 3271, 3272, 3273, 3274, 3275, 3276, 3277
NCBI Taxonomy refs (TaxIDs): 573Reference(s) to other database(s): GTC:G57429IZ
Show glycosyltransferases
NMR conditions: in D2O at 323 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
3,3,2,2 aLRhap 100.5 76.5 70.6 73.0 70.1 17.5
3,3,2 aLRhap 101.6 79.2 70.6 73.0 70.1 17.5
3,3 aLRhap 101.6 78.6 70.9 73.0 70.7 17.5
3 bDGalp 105.2 72.0 80.4 69.5 75.9 61.9
4 aLRhap 100.7 71.3 70.8 73.2 69.9 17.5
aDGalpA 98.2 68.2 80.4 77.4 72.6 175.5
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
3,3,2,2 aLRhap 5.10 4.14 3.88 3.51 3.75 1.29
3,3,2 aLRhap 5.14 4.11 3.88 3.48 3.72 1.28
3,3 aLRhap 5.18 4.08 3.95 3.50 3.81 1.30
3 bDGalp 4.65 3.72 3.72 4.00 3.71 3.75-3.87
4 aLRhap 5.40 4.06 3.81 3.37 3.79 1.23
aDGalpA 5.09 4.08 4.21 4.67 4.65 -
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
3,3,2,2 aLRhap 100.5/5.10 76.5/4.14 70.6/3.88 73.0/3.51 70.1/3.75 17.5/1.29
3,3,2 aLRhap 101.6/5.14 79.2/4.11 70.6/3.88 73.0/3.48 70.1/3.72 17.5/1.28
3,3 aLRhap 101.6/5.18 78.6/4.08 70.9/3.95 73.0/3.50 70.7/3.81 17.5/1.30
3 bDGalp 105.2/4.65 72.0/3.72 80.4/3.72 69.5/4.00 75.9/3.71 61.9/3.75-3.87
4 aLRhap 100.7/5.40 71.3/4.06 70.8/3.81 73.2/3.37 69.9/3.79 17.5/1.23
aDGalpA 98.2/5.09 68.2/4.08 80.4/4.21 77.4/4.67 72.6/4.65
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 3,3,2,2 | aLRhap | 5.10 | 4.14 | 3.88 | 3.51 | 3.75 | 1.29 |
| 3,3,2 | aLRhap | 5.14 | 4.11 | 3.88 | 3.48 | 3.72 | 1.28 |
| 3,3 | aLRhap | 5.18 | 4.08 | 3.95 | 3.50 | 3.81 | 1.30 |
| 3 | bDGalp | 4.65 | 3.72 | 3.72 | 4.00 | 3.71 | 3.75
3.87 |
| 4 | aLRhap | 5.40 | 4.06 | 3.81 | 3.37 | 3.79 | 1.23 |
| | aDGalpA | 5.09 | 4.08 | 4.21 | 4.67 | 4.65 |
|
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 3,3,2,2 | aLRhap | 100.5 | 76.5 | 70.6 | 73.0 | 70.1 | 17.5 |
| 3,3,2 | aLRhap | 101.6 | 79.2 | 70.6 | 73.0 | 70.1 | 17.5 |
| 3,3 | aLRhap | 101.6 | 78.6 | 70.9 | 73.0 | 70.7 | 17.5 |
| 3 | bDGalp | 105.2 | 72.0 | 80.4 | 69.5 | 75.9 | 61.9 |
| 4 | aLRhap | 100.7 | 71.3 | 70.8 | 73.2 | 69.9 | 17.5 |
| | aDGalpA | 98.2 | 68.2 | 80.4 | 77.4 | 72.6 | 175.5 |
|
There is only one chemically distinct structure:
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Found 1 record.
Displayed record 1
