Taxonomic group: bacteria / Fusobacteria
(Phylum: Fusobacteria)
Host organism: Homo sapiens
Associated disease: periodontitis [ICD11:
DA0C 
];
infection due to Fusobacterium nucleatum [ICD11:
XN4P8 ]
The structure was elucidated in this paperNCBI PubMed ID: 32396022Publication DOI: 10.1139/cjm-2020-0117Journal NLM ID: 0372707Publisher: National Research Council of Canada
Correspondence: Andrew D. Cox < Andrew.Cox
nrc-cnrc.gc.ca>
Institutions: Vaccine and Emerging Infections Research, Human Health Therapeutics Research Centre, National Research Council, Ottawa, ON K1A 0R6, Canada
Fusobacterium nucleatum is becoming increasingly recognised as an emerging pathogen, gaining attention as a potential factor for exacerbating colorectal cancer and is strongly linked with pregnancy complications including pre-term and still births. Little is known about the virulence factors of this organism; thus, we have initiated studies to examine the bacterium's surface glycochemistry. In an effort to characterise the surface carbohydrates of F. nucleatum, the aims of this study were to investigate the structure of the lipopolysaccharide (LPS) O-antigen of the cancer-associated isolate F. nucleatum strain CC 7/3 JVN3 C1 (hereafter C1) and to develop monoclonal antibodies (mAbs) to the LPS O-antigen that may be beneficial to the growing field of F. nucleatum research. In this study, we combined several technologies, including nuclear magnetic resonance (NMR) spectroscopy, to elucidate the structure of the LPS O-antigen repeat unit as -[-4-β-Gal-3-α-FucNAc4N-4-α-NeuNAc-]-. We have previously identified this structure as the LPS O-antigen repeat unit from strain 10953. In this present study, we developed a mAb to the C1 LPS O-antigen and confirmed the mAbs cross-reactivity to the 10953 strain, thus confirming the structural identity.
Lipopolysaccharide, monoclonal antibody, serotyping, vaccine, Fusobacterium nucleatum, anticorps monoclonal, serotypage
Structure type: polymer chemical repeating unit
Location inside paper: abstract, table 1, polysaccharide
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_136044,IEDB_136794,IEDB_137472,IEDB_141794,IEDB_142345,IEDB_146100,IEDB_149174,IEDB_190606,SB_165,SB_166,SB_170,SB_171,SB_172,SB_187,SB_195,SB_7,SB_84,SB_88
Methods: 13C NMR, 1H NMR, NMR-2D, sugar analysis, ELISA, mild acid hydrolysis, GPC, immunization
Comments, role: the NMR solution was not indicated;
Related record ID(s): 3281
NCBI Taxonomy refs (TaxIDs): 851Reference(s) to other database(s): GTC:G68790OG
Show glycosyltransferases
NMR conditions: at 298 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6 C7 C8 C9
4,3 bDGalp 106.2 72.1 72.8 73.4 76.3 62.2
4,2 Ac
4 aDFucpN4N 95.3 48.8 75.7 56.1 64.0 16.7
5 Ac
aXNeup ? ? 37.9 75.5 50.3 74.0 68.9 72.9 63.9
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6 H7 H8 H9
4,3 bDGalp 4.50 3.52 3.61 4.49 3.70 3.66-3.72
4,2 Ac
4 aDFucpN4N 5.04 4.23 4.11 3.93 4.18 1.33
5 Ac
aXNeup - - 1.78-2.82 3.69 4.04 3.72 3.63 3.85 3.63-3.86
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6 C7/H7 C8/H8 C9/H9
4,3 bDGalp 106.2/4.50 72.1/3.52 72.8/3.61 73.4/4.49 76.3/3.70 62.2/3.66-3.72
4,2 Ac
4 aDFucpN4N 95.3/5.04 48.8/4.23 75.7/4.11 56.1/3.93 64.0/4.18 16.7/1.33
5 Ac
aXNeup 37.9/1.78-2.82 75.5/3.69 50.3/4.04 74.0/3.72 68.9/3.63 72.9/3.85 63.9/3.63-3.86
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 | H7 | H8 | H9 |
|---|
| 4,3 | bDGalp | 4.50 | 3.52 | 3.61 | 4.49 | 3.70 | 3.66
3.72 | |
| 4,2 | Ac | |
| 4 | aDFucpN4N | 5.04 | 4.23 | 4.11 | 3.93 | 4.18 | 1.33 | |
| 5 | Ac | |
| | aXNeup |
|
| 1.78
2.82 | 3.69 | 4.04 | 3.72 | 3.63 | 3.85 | 3.63
3.86 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 | C7 | C8 | C9 |
|---|
| 4,3 | bDGalp | 106.2 | 72.1 | 72.8 | 73.4 | 76.3 | 62.2 | |
| 4,2 | Ac | |
| 4 | aDFucpN4N | 95.3 | 48.8 | 75.7 | 56.1 | 64.0 | 16.7 | |
| 5 | Ac | |
| | aXNeup | ? | ? | 37.9 | 75.5 | 50.3 | 74.0 | 68.9 | 72.9 | 63.9 |
|
![](nmr_drawer.py?width=750&height=130&nucleus=C&rotate_labels=false&spectrum=[{"s":106.2,"l":"1","c":[0,0,225]},{"s":72.1,"l":"2","c":[0,0,225]},{"s":72.8,"l":"3","c":[0,0,225]},{"s":73.4,"l":"4","c":[0,0,225]},{"s":76.3,"l":"5","c":[0,0,225]},{"s":62.2,"l":"6","c":[0,0,225]},{"s":95.3,"l":"1","c":[230,0,30]},{"s":48.8,"l":"2","c":[230,0,30]},{"s":75.7,"l":"3","c":[230,0,30]},{"s":56.1,"l":"4","c":[230,0,30]},{"s":64,"l":"5","c":[230,0,30]},{"s":16.7,"l":"6","c":[230,0,30]},{"s":37.9,"l":"3","c":[0,165,0]},{"s":75.5,"l":"4","c":[0,165,0]},{"s":50.3,"l":"5","c":[0,165,0]},{"s":74,"l":"6","c":[0,165,0]},{"s":68.9,"l":"7","c":[0,165,0]},{"s":72.9,"l":"8","c":[0,165,0]},{"s":63.9,"l":"9","c":[0,165,0]}])
The spectrum also has 2 signals at unknown positions (not plotted). |
There is only one chemically distinct structure:
Found 
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