Landersjo C, Weintraub A, Widmalm G Structural analysis of the O-antigen polysaccharide from the Shiga toxin-producing Escherichia coli O172 European Journal of Biochemistry268(8) (2001)
2239-2245
The structure was elucidated in this paper NCBI PubMed ID:11298740 Journal NLM ID:0107600 Publisher: Oxford, UK: Blackwell Science Ltd. on behalf of the Federation of European Biochemical Societies Correspondence: G. Widmalm <gworgan.su.se> Institutions: Department of Organic Chemistry, Arrhenius Laboratory, Stockholm University, Sweden, Karolinska Institute, Department of Microbiology, Pathology and Immunology, Division of Clinical Bacteriology, Huddinge University Hospital, Sweden
The structure of the O-antigen polysaccharide from Escherichia coli O172 has been determined. In combination with sugar analysis, NMR spectroscopy shows that the polysaccharide is composed of pentasaccharide repeating units. Sequential information was obtained by mass spectrometry and two-dimensional NMR techniques. An O-acetyl group was present as 0.7 equivalent per repeating unit. Treatment of the O-deacetylated polysaccharide with aqueous 48% hydrofluoric acid rendered cleavage of the phosphodiester in the backbone of the polymer and the pentasaccharide isolated after gel permeation chromatography was structurally characterized. Subsequent NMR experiments on polymeric materials revealed the structure of the repeating unit of the O-polysaccharide from E. coli O172 as: →P-4)-α-D-Glcp-(1→3)-α-L-FucpNAc-(1→3)-α-D-GlcpNAc-(1→3)-α-L-FucpNAc-(1→4)-α-D-Glcp6Ac-(1→.
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