Taxonomic group: bacteria / Firmicutes
(Phylum: Firmicutes)
Associated disease: diarrhea [ICD11:
ME05.1 
, ICD11:
SA55 ]
The structure was elucidated in this paperNCBI PubMed ID: 23602537Publication DOI: 10.1016/j.vaccine.2013.04.018Journal NLM ID: 8406899Publisher: Elsevier
Correspondence: Mario A. Monteiro <monteiro
uoguelph.ca>
Institutions: Department of Chemistry, University of Guelph, Guelph, ON N1G 2W1, Canada, Stellar Biotechnologies Inc., Port Hueneme, CA, USA, Department of Molecular and Cellular Biology, University of Guelph, Guelph, ON, Canada
Constipation and diarrhea are common in autistic patients. Treatment with antibiotics against bacteria appears to partially alleviate autistic-related symptoms. Clostridium bolteae is a bacterium that has been shown to be overabundant in the intestinal tract of autistic children suffering from gastric intestinal ailments, and as such is an organism that could potentially aggravate gastrointestinal symptoms. We set out to investigate the cell-wall polysaccharides of C. bolteae in order to evaluate their structure and immunogenicity. Our explorations revealed that C. bolteae produces a conserved specific capsular polysaccharide comprised of rhamnose and mannose units: [→3)-α-D-Manp-(1→4)-β-d-Rhap-(1→], which is immunogenic in rabbits. These findings are the first description of a C. bolteae immunogen and indicate the prospect of using this polysaccharide as a vaccine to reduce or prevent C. bolteae colonization of the intestinal tract in autistic patients, and as a diagnostic marker for the rapid detection of C. bolteae in a clinical setting.
polysaccharide, diarrhea, vaccine, diagnostic, Clostridium bolteae, Regressive-autism
Structure type: polymer chemical repeating unit
Location inside paper: abstract, p.2789, table S1
Compound class: CPS
Contained glycoepitopes: IEDB_130701,IEDB_1394181,IEDB_144983,IEDB_152206,IEDB_983930,SB_44,SB_67,SB_72
Methods: 13C NMR, 1H NMR, NMR-2D, GC-MS, sugar analysis, MD simulations, immunological assays
Comments, role: published erroneous NMR spectra of #4_aDManp (13C: 111.1 76.8 83.4 72.4 65.3 65.2-65.3; 1H: 5.30 4.24 4.13 3.72 3.83 3.71-3.82) was removed by CSDB staff
NCBI Taxonomy refs (TaxIDs): 208479Reference(s) to other database(s): GTC:G64150GO
Show glycosyltransferases
NMR conditions: in D2O at 298 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
4 aDManp
bDRhap 103.6 73.2 72.8 82.1 70.6 19.8
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
4 aDManp
bDRhap 4.90 3.99 3.92 3.55 3.93 1.34
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
4 aDManp
bDRhap 103.6/4.90 73.2/3.99 72.8/3.92 82.1/3.55 70.6/3.93 19.8/1.34
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 4 | aDManp | |
| | bDRhap | 4.90 | 3.99 | 3.92 | 3.55 | 3.93 | 1.34 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 4 | aDManp | |
| | bDRhap | 103.6 | 73.2 | 72.8 | 82.1 | 70.6 | 19.8 |
|
There is only one chemically distinct structure:
Found 
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(later renamed to: Enterocloster bolteae 16351)