Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Host organism: Oncorhynchus mykiss
Organ / tissue: gillsAssociated disease: motile aeromonad septicemia (MAS)
The structure was elucidated in this paperNCBI PubMed ID: 32504970Publication DOI: 10.1016/j.carres.2020.108036Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: aturska
hektor.umcs.lublin.pl
Institutions: Department of Genetics and Microbiology, M. Curie-Sklodowska University, Akademicka 19, 20-033, Lublin, Poland, Department of Fish Diseases, National Veterinary Research Institute, Partyzantow 57, 24-100, Pulawy, Poland
The O-specific polysaccharide (OPS) was isolated from the lipopolysaccharide of Aeromonas veronii bv. sobria strain Pt393, which is pathogenic to the rainbow trout (Oncorhynchus mykiss), after mild acid hydrolysis followed by GPC. The high-molecular-weight OPS fraction was studied with chemical methods, mass spectrometry, and 1H and 13C NMR spectroscopy techniques, including 2D 1H,1H COSY, TOCSY, NOESY, 1H-detected heteronuclear 1H,13C HSQC, and HMBC experiments. It was found that the O-specific polysaccharide was built of a tetrasaccharide repeating unit composed of α-GalpNAc, α-FucpNAc, β-QuipNAc, and α-Fucp4NAc (4-acetamido-4,6-dideoxy-d-galactose, tomosamine) residues. The following structure of the OPS of A. sobria strain Pt393 was established: →4)-α-D-GalpNAc-(1→3)-α-L-FucpNAc-(1→3)-β-D-QuipNAc-(1→3)-α-D-Fucp4NAc-(1→.
LPS, O-specific polysaccharide, 6-dideoxy-D-galactose, 4-Acetamido-4, Fish pathogen, Aeromonas veronii bv.sobria, Tomosamine
Structure type: polymer chemical repeating unit
Location inside paper: abstract, table 1
Compound class: O-polysaccharide
Contained glycoepitopes: IEDB_130648,IEDB_137473,IEDB_1391961,IEDB_141584,IEDB_885822
Methods: 13C NMR, 1H NMR, methylation, GLC-MS, NMR-2D, SDS-PAGE, sugar analysis, GLC, methanolysis, GPC, acetylation
NCBI Taxonomy refs (TaxIDs): 114517Reference(s) to other database(s): GTC:G57380ER
Show glycosyltransferases
NMR conditions: in D2O at 305 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
3,3,3,2 Ac 175.6 23.5
3,3,3 aDGalpN 99.7 51.8 68.3 79.7 73.2 61.9
3,3,2 Ac 175.0 23.6
3,3 aLFucpN 98.8 50.0 74.4 72.3 67.9 16.4
3,2 Ac 175.3 23.4
3 bDQuipN 103.0 57.2 79.2 75.2 73.2 18.2
4 Ac 176.0 23.3
aDFucp4N
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
3,3,3,2 Ac - 2.07
3,3,3 aDGalpN 5.14 4.25 4.06 4.07 4.07 3.83-3.91
3,3,2 Ac - 2.02
3,3 aLFucpN 5.00 4.31 4.05 3.94 4.49 1.19
3,2 Ac - 1.98
3 bDQuipN 4.66 3.86 3.63 3.23 3.49 1.32
4 Ac - 2.09
aDFucp4N 4.91 3.84 4.12 4.37 4.52 1.03
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
3,3,3,2 Ac 23.5/2.07
3,3,3 aDGalpN 99.7/5.14 51.8/4.25 68.3/4.06 79.7/4.07 73.2/4.07 61.9/3.83-3.91
3,3,2 Ac 23.6/2.02
3,3 aLFucpN 98.8/5.00 50.0/4.31 74.4/4.05 72.3/3.94 67.9/4.49 16.4/1.19
3,2 Ac 23.4/1.98
3 bDQuipN 103.0/4.66 57.2/3.86 79.2/3.63 75.2/3.23 73.2/3.49 18.2/1.32
4 Ac 23.3/2.09
aDFucp4N NMR TSV error 2: unequal length of 13C and 1H datasets
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 3,3,3,2 | Ac |
| 2.07 | |
| 3,3,3 | aDGalpN | 5.14 | 4.25 | 4.06 | 4.07 | 4.07 | 3.83
3.91 |
| 3,3,2 | Ac |
| 2.02 | |
| 3,3 | aLFucpN | 5.00 | 4.31 | 4.05 | 3.94 | 4.49 | 1.19 |
| 3,2 | Ac |
| 1.98 | |
| 3 | bDQuipN | 4.66 | 3.86 | 3.63 | 3.23 | 3.49 | 1.32 |
| 4 | Ac |
| 2.09 | |
| | aDFucp4N | 4.91 | 3.84 | 4.12 | 4.37 | 4.52 | 1.03 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 3,3,3,2 | Ac | 175.6 | 23.5 | |
| 3,3,3 | aDGalpN | 99.7 | 51.8 | 68.3 | 79.7 | 73.2 | 61.9 |
| 3,3,2 | Ac | 175.0 | 23.6 | |
| 3,3 | aLFucpN | 98.8 | 50.0 | 74.4 | 72.3 | 67.9 | 16.4 |
| 3,2 | Ac | 175.3 | 23.4 | |
| 3 | bDQuipN | 103.0 | 57.2 | 79.2 | 75.2 | 73.2 | 18.2 |
| 4 | Ac | 176.0 | 23.3 | |
| | aDFucp4N | |
|
There is only one chemically distinct structure:
Found 
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