Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: nosocomial infections [ICD11:
XB25 
];
infection due to Enterobacter cloacae [ICD11:
XN3YM ]
NCBI PubMed ID: 32411106Publication DOI: 10.3389/fmicb.2020.00727Journal NLM ID: 101548977Publisher: Lausanne: Frontiers Research Foundation
Correspondence: Tao Han <hantaomd
126.com>; Xi Guo <guoxi
nankai.edu.cn>
Institutions: The Third Central Hospital of Tianjin, Tianjin Key Laboratory of Extracorporeal Life Support for Critical Diseases, Tianjin Institute of Hepatobiliary Disease, Tianjin, China, Department of Vascular Surgery, Tianjin Hospital, Tianjin, China, Tianjin Children's Hospital, Third Central Clinical College of Tianjin Medical University, Tianjin, China, Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, TEDA Institute of Biological Sciences and Biotechnology, Nankai University, Tianjin, China
Enterobacter cloacae is a well-characterized opportunistic pathogen that is closely associated with various nosocomial infections. The O-antigen, which is one of the most variable constituents on the cell surface, has been used widely and traditionally for serological classification of many gram-negative bacteria. E. cloacae is divided into 30 serotypes, based on its O-antigen diversity. In this study, by using genomic and comparative-genomic approaches, we analyzed the O-antigen gene clusters of 26 E. cloacae serotypes in depth. We also identified the sero-specific gene for each serotype and developed a multiplex polymerase chain reaction (PCR) method. The sensitivity of the assay was 0.1 ng for genomic DNA and 10(3) colony forming units for pure cultures. The assay reliability was evaluated by double-blinded testing with 81 clinical strains. Furthermore, we established a valid, genome-based tool for in silico serotyping of E. cloacae. By screening 431 E. cloacae genomes deposited in GenBank, 304 were classified into current antigenic scheme, and 112 were allocated into 55 putative novel serotypes. Our results represent the first genetic basis of the O-antigen diversity and variation of E. cloacae, providing a rationale for studying the O-antigen associated evolution and pathogenesis of this bacterium. In addition, we extended the current serotyping system for E. cloacae, which is important for detection and epidemiological surveillance purposes for this important pathogen.
serotype, O-antigen, gene cluster, Enterobacter cloacae, multiplex PCR
Structure type: polymer chemical repeating unit
Location inside paper: Fig.4A
The structure in this paper was incorrect:
Compound class: O-polysaccharide, O-antigen, LPS
Contained glycoepitopes: IEDB_130701,IEDB_136104,IEDB_137485,IEDB_140116,IEDB_141830,IEDB_142488,IEDB_143632,IEDB_144983,IEDB_144998,IEDB_146664,IEDB_152206,IEDB_983930,IEDB_983931,SB_136,SB_192,SB_196,SB_44,SB_67,SB_72
Methods: PCR, bioinformatic analysis, serotyping analysis, sequencing
Comments, role: structure from ref. Moule et al., 1989
Related record ID(s): 5128, 5129, 5130, 5131
NCBI Taxonomy refs (TaxIDs): 550Reference(s) to other database(s): GTC:G21094FS, CCSD:
3710, CBank-STR:14172
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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