Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Escherichia coli [ICD11:
XN6P4 
]
The structure was elucidated in this paperNCBI PubMed ID: 9208951Publication DOI: 10.1111/j.1432-1033.1997.00565.xJournal NLM ID: 0107600Publisher: Oxford, UK: Blackwell Science Ltd. on behalf of the Federation of European Biochemical Societies
Institutions: Department of Organic Chemistry, Arrhenius Laboratory, Stockholm University, Sweden, Swedish Insitute of Infectious Disease Control (SMI), Stockholm, Sweden
The structure of the O-antigenic polysaccharide from Escherichia coli O167:H5 has been investigated. Sugar and methylation analyses, fast-atom-bombardment mass spectrometry and 1H- and 13C NMR spectroscopy were the main methods used. The structure of the repeating unit of the polysaccharide was found to be: [formula in text]. Oligosaccharide derivatives of the polysaccharide were obtained by HF solvolysis and by a Smith degradation. Furthermore, base treatment of the polysaccharide led to a degraded polymeric material. For the methylated polysaccharide the amide linkage between alanine and the galacturonic acid residue was reductively cleaved with LiBD4 in ethanol, to give, among other things, a 3-O-methyl galactose derivative.
Lipopolysaccharide, NMR, LPS, structure, structural, polysaccharide, O-antigen, O antigen, Escherichia, Escherichia coli, polysaccharides, structural studies, galactofuranose, galacturonic acid, amide, L-alanine
Structure type: oligomer
Location inside paper: p. 568
Contained glycoepitopes: IEDB_114709,IEDB_135813,IEDB_136907,IEDB_137340,IEDB_141807,IEDB_151531
Methods: methylation, NMR-2D, FAB-MS, partial acid hydrolysis, NMR, sugar analysis, Smith degradation
Comments, role: Smith degradation product
Related record ID(s): 3158, 3229, 3231
NCBI Taxonomy refs (TaxIDs): 562Reference(s) to other database(s): GlycomeDB:
25946
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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