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Taxonomic group: bacteria / Proteobacteria (Phylum: Proteobacteria)
Associated disease: infection due to Enterobacter cloacae [ICD11: XN3YM

]

The structure was elucidated in this paper
NCBI PubMed ID: 23218327
Publication DOI: 10.1016/j.carbpol.2012.10.014
Journal NLM ID: 8307156
Publisher: Elsevier
Correspondence: Yizhen Wang <yzwang

zju.edu.cn>; yzwang321

zju.edu.cn
Institutions: College of Animal Science, Zhejiang University, Key Laboratory of Animal Nutrition and Feed Science, Ministry of Agriculture (East China), Zhejiang Provincial Laboratory of Feed and Animal Nutrition, Hangzhou, China

A novel high molecular weight (1.1 × 10(6)Da) exopolysaccharide (EPS) produced by Enterobacter cloacae Z0206 strain was isolated by column chromatography. Complete hydrolysis of the EPS followed by gas chromatography mass spectrometry (GC-MS) and high performance liquid chromatography (HPLC) analyses showed that the EPS is composed of L-fucose, D-glucose, D-galactose, D-glucuronic acid and pyruvic acid in the approximate molar ratio of 2:1:3:1:1. Partial acid hydrolysis of the purified EPS followed by gel permeation chromatography (GPC) yielded a hexasaccharide. A combination of chemical analysis coupled with mass spectrometry and 1D and 2D NMR spectroscopy applied to the oligosaccharide showed that the EPS comprises a heptasaccharide repeating unit.

hexasaccharide, NMR spectroscopy, structural analysis, mass spectrometry, Enterobacter cloacae Z0206

Structure type: polymer chemical repeating unit ; 1100000
Location inside paper: p.509, fig.6
Compound class: EPS
Contained glycoepitopes: IEDB_115136,IEDB_136044,IEDB_136045,IEDB_136906,IEDB_137472,IEDB_140630,IEDB_141794,IEDB_142488,IEDB_142489,IEDB_144562,IEDB_146664,IEDB_151528,IEDB_151770,IEDB_152214,IEDB_174333,IEDB_190606,IEDB_423153,IEDB_983931,SB_165,SB_166,SB_187,SB_192,SB_195,SB_7,SB_86,SB_88

Methods: 13C NMR, 1H NMR, methylation, periodate oxidation, NMR-2D, partial acid hydrolysis, GC-MS, sugar analysis, ESI-MS, acid hydrolysis, Smith degradation, HPLC, GPC

Related record ID(s): 3326
NCBI Taxonomy refs (TaxIDs): 550
Show glycosyltransferases

There is only one chemically distinct structure:

*O[C@@H]1[C@@H](O)[C@H](O[C@@H]2[C@H](C)O[C@@H](O[C@@H]3[C@H](C)O[C@@H](O[C@H]4[C@@H](CO)O[C@@H](*)[C@H](O)[C@H]4O)[C@@H](O)[C@@H]3O[C@@H]3O[C@H](CO)[C@@H](O)[C@H](O[C@@H]4O[C@H](C(=O)O)[C@@H](O[C@H]5O[C@@H]6CO[C@@](C)(C(=O)O)O[C@@H]6[C@H](O)[C@H]5O)[C@H](O)[C@H]4O)[C@H]3O)[C@@H](O)[C@@H]2O)O[C@H](CO)[C@@H]1O

SVG MW SMILES 3D mol Ionize