Taxonomic group: bacteria / Firmicutes
(Phylum: Firmicutes)
Associated disease: infection due to Streptococcus pneumoniae [ICD11:
XN3PW 
]
NCBI PubMed ID: 32152541Publication DOI: 10.1038/s41589-020-0494-0Journal NLM ID: 101231976Publisher: New York, NY: Nature Publishing Group
Correspondence: mkimber
uoguelph.ca; cwhitfie
uoguelph.ca
Institutions: Department of Chemistry, University of Alberta, Edmonton, AB, Canada, Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario, Canada
Lipopolysaccharide O-antigen is an attractive candidate for immunotherapeutic strategies targeting antibiotic-resistant Klebsiella pneumoniae. Several K. pneumoniae O-serotypes are based on a shared O2a-antigen backbone repeating unit: (→3)-α-Galp-(1→3)-β-Galf-(1→). O2a antigen is synthesized on undecaprenol diphosphate in a pathway involving the O2a polymerase, WbbM, before its export by an ATP-binding cassette transporter. This dual domain polymerase possesses a C-terminal galactopyranosyltransferase resembling known GT8 family enzymes, and an N-terminal DUF4422 domain identified here as a galactofuranosyltransferase defining a previously unrecognized family (GT111). Functional assignment of DUF4422 explains how galactofuranose is incorporated into various polysaccharides of importance in vaccine production and the food industry. In the 2.1-Å resolution structure, three WbbM protomers associate to form a flattened triangular prism connected to a central stalk that orients the active sites toward the membrane. The biochemical, structural and topological properties of WbbM offer broader insight into the mechanisms of assembly of bacterial cell-surface glycans.
Lipopolysaccharide, O-antigen, mechanism, galactofuranose, glycosyltransferase, Klebsiella pneumoniae, O-antigen polymerase, vaccine, transporter, glycans, O-serotypes, undecaprenol
Structure type: polymer chemical repeating unit
Location inside paper: Fig.S7, S. pneumoniae 676/67
Compound class: CPS
Contained glycoepitopes: IEDB_114703,IEDB_136095,IEDB_136906,IEDB_137472,IEDB_141794,IEDB_142488,IEDB_144998,IEDB_146664,IEDB_151528,IEDB_190606,IEDB_591403,IEDB_983931,SB_192,SB_7
Methods: 13C NMR, 1H NMR, gel filtration, NMR-2D, PCR, SDS-PAGE, DNA techniques, ESI-MS, biochemical methods, bioinformatic analysis, enzyme structure, topological information
3D data: 3D data
Related record ID(s): 3328, 3329, 3330, 3331, 3332, 3333, 3334, 3335, 3337, 32083
NCBI Taxonomy refs (TaxIDs): 1313Reference(s) to other database(s): GTC:G89467XD
Show glycosyltransferases
There is only one chemically distinct structure:
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