Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Escherichia coli [ICD11:
XN6P4 
]
The structure was elucidated in this paperNCBI PubMed ID: 9213433Publication DOI: 10.1139/o96-025Journal NLM ID: 8606068Publisher: Ottawa: National Research Council of Canada
Institutions: Institute for Biological Sciences, National Research Council of Canada, Ottava, Canada
The structure of the O-polysaccharide component of the lipopolysaccharide produced by Escherichia coli 017 (ATCC 23512) was determined by the use of methylation, periodate oxidation, one- and two-dimensional nuclear magnetic resonance spectroscopy, and mass spectrometric methods. The O-polysaccharide was found to be a high molecular weight polymer of repeating branched pentasaccharide units composed of D-mannose, D-glucose, and 2-acetamido-2-deoxy-D-glucose residues (3:1:1) and had the structure (formula: see text).
Lipopolysaccharide, LPS, structural, characterization, polysaccharide, serotype, Escherichia, Escherichia coli, O-antigenic, O-antigenic polysaccharide
Structure type: suggested polymer biological repeating unit
Location inside paper: abstract
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_130701,IEDB_136104,IEDB_137340,IEDB_137485,IEDB_140116,IEDB_141807,IEDB_141830,IEDB_142488,IEDB_143632,IEDB_144983,IEDB_144998,IEDB_146664,IEDB_151531,IEDB_152206,IEDB_983930,IEDB_983931,SB_136,SB_192,SB_196,SB_44,SB_67,SB_72
Methods: NMR
Comments, role: chemical repeat frame is different in the paper
Related record ID(s): 20644, 21693, 23197, 30343
NCBI Taxonomy refs (TaxIDs): 1010800Reference(s) to other database(s): GTC:G77194DN, GlycomeDB:
37032
Show glycosyltransferases
NMR conditions: in D2O at 300 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
3,2,2 aDManp 103.51 70.83 71.60 66.7 72.37 65.70
3,2 aDManp 100.23 79.18 70.83 67.37 73.43 61.63
3 bDManp 100.95 76.52 74.75 67.53 77.67 61.75
2 Ac
6 aDGlcp 98.59 72.37 73.95 70.36 72.68 61.32
aDGlcpN 97.85 53.77 81.29 69.14 71.14 66.32
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
3,2,2 aDManp 5.04 4.11 3.85 3.96 3.82 3.53-4.14
3,2 aDManp 5.32 4.07 4.03 3.76 4.00 3.82-3.86
3 bDManp 4.76 3.59 3.72 3.63 3.40 3.74-3.93
2 Ac
6 aDGlcp 4.96 3.55 3.73 3.43 3.71 3.77-3.84
aDGlcpN 4.88 4.11 3.94 3.64 3.93 3.76-4.00
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
3,2,2 aDManp 103.51/5.04 70.83/4.11 71.60/3.85 66.7/3.96 72.37/3.82 65.70/3.53-4.14
3,2 aDManp 100.23/5.32 79.18/4.07 70.83/4.03 67.37/3.76 73.43/4.00 61.63/3.82-3.86
3 bDManp 100.95/4.76 76.52/3.59 74.75/3.72 67.53/3.63 77.67/3.40 61.75/3.74-3.93
2 Ac
6 aDGlcp 98.59/4.96 72.37/3.55 73.95/3.73 70.36/3.43 72.68/3.71 61.32/3.77-3.84
aDGlcpN 97.85/4.88 53.77/4.11 81.29/3.94 69.14/3.64 71.14/3.93 66.32/3.76-4.00
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 3,2,2 | aDManp | 5.04 | 4.11 | 3.85 | 3.96 | 3.82 | 3.53
4.14 |
| 3,2 | aDManp | 5.32 | 4.07 | 4.03 | 3.76 | 4.00 | 3.82
3.86 |
| 3 | bDManp | 4.76 | 3.59 | 3.72 | 3.63 | 3.40 | 3.74
3.93 |
| 2 | Ac | |
| 6 | aDGlcp | 4.96 | 3.55 | 3.73 | 3.43 | 3.71 | 3.77
3.84 |
| | aDGlcpN | 4.88 | 4.11 | 3.94 | 3.64 | 3.93 | 3.76
4.00 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 3,2,2 | aDManp | 103.51 | 70.83 | 71.60 | 66.7 | 72.37 | 65.70 |
| 3,2 | aDManp | 100.23 | 79.18 | 70.83 | 67.37 | 73.43 | 61.63 |
| 3 | bDManp | 100.95 | 76.52 | 74.75 | 67.53 | 77.67 | 61.75 |
| 2 | Ac | |
| 6 | aDGlcp | 98.59 | 72.37 | 73.95 | 70.36 | 72.68 | 61.32 |
| | aDGlcpN | 97.85 | 53.77 | 81.29 | 69.14 | 71.14 | 66.32 |
|
There is only one chemically distinct structure:
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