Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Campylobacter jejuni [ICD11:
XN4Q5 
]
The structure was elucidated in this paperNCBI PubMed ID: 12433486Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: angela.savage
nuigalway.ie
Institutions: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, Department of Microbiology, National University of Ireland, Galway, Ireland, Department of Chemistry, National University of Ireland, Galway, Ireland
Campylobacter jejuni 81116 has been extensively investigated in studies on genes associated with the synthesis of Campylobacter lipopoly/lipooligosaccharides (LPS/LOS). Despite these investigations, data on the chemical structure of polysaccharides from C. jejuni 81116 have been absent. The present study was undertaken to fill that void. Biomass was grown in large quantities on agar medium, harvested and extracted by hot phenol-water extraction. Subsequently, extracts were treated by DNase, RNase and proteinase K to remove contaminants. After mild acid treatment, followed by preparative gel-permeation and anion- exchange chromatography, fractions were isolated and studied by 1H and 13C NMR spectroscopy, including 2D COSY, TOCSY, 1H,(13)C HMQC and HMBC experiments. These advanced investigations revealed the occurrence of two different polysaccharides in the approximate ratio of 3:1, each having a tetrasaccharide repeating unit. Polysaccharide A contained glucose, glucuronic acid and mannose, and is O-acetylated. Polysaccharide B contained glucose, galactose and N-acetylglucosamine. Importantly, polysaccharide A is acidic, whereas polysaccharide B is neutral
Campylobacter jejuni, polysaccharide structure, NMR abstract
Structure type: polymer chemical repeating unit
Location inside paper: structure A
Trivial name: acidic polysacharide
Compound class: CPS
Contained glycoepitopes: IEDB_115136,IEDB_130701,IEDB_140630,IEDB_142488,IEDB_144983,IEDB_144998,IEDB_146664,IEDB_152206,IEDB_153755,IEDB_983930,IEDB_983931,SB_192,SB_44,SB_67,SB_72
Methods: NMR-2D, NMR, anion-exchange chromatography
Related record ID(s): 3813, 3894, 20076
NCBI Taxonomy refs (TaxIDs): 197Reference(s) to other database(s): GTC:G17069XI
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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