A recently described water-soluble exopolysaccharide of Burkholderia pseudomallei recognized by the IgG 1 monoclonal antibody 3015 [Steinmetz, I., Rohde, M. & Brenneke, B. (1995) Infect. Immun. 63, 3959-3965] was isolated by repetitive ethanol-precipitation steps and by anion-exchange chromatography. The structure of the polysaccharide was determined by a combination of chemical-derivatization and mass-spectrometric techniques (compositional and methylation analysis, GC/MS, and electrospray-ionization-MS/MS of reduced and permethylated hydrolytic fragments), and two-dimensional 1H NMR methods (COSY, TOCSY and NOESY) and confirmed by isolation and structural characterization of the depolymerized repeating unit of the polysaccharide. The combined structural data established a linear tetrasaccharide repeating unit consisting of three galactose residues, one bearing a 2-linked O-acetyl group, and a 3-deoxy-D-manno-2-octulosonic acid residue. [→3)-β-D-Galp2Ac-(1→4)-α-D-Galp-(1→3)-β-D-Galp-(1→5)-β-Kdo-(2→]n.

structure, Burkholderia, acidic, Kdo, Burkholderia pseudomallei, exopolysaccharide, acidic exopolysaccharide

13C NMR data:
missing...
1H NMR data:
Linkage	Residue	H1	H2	H3	H4	H5	H6	H7	H8
5,3,4	bDGalp	4.602	3.590	3.675	3.908	3.67-3.86	3.67-3.86
5,3	aDGalp	5.146	3.940	4.064	4.270	3.67-3.86	3.67-3.86
5	bDGalp	4.719	3.77	3.77	4.155	3.67-3.86	3.76-3.86
	?XKdo?	-	-	1.922-2.111	4.157	4.260	3.83	4.014	3.628-3.830

There are 2 chemically distinct structures. Please, select:
bDGalp(1-4)aDGalp(1-3)bDGalp(1-5)?XKdop bDGalp(1-4)aDGalp(1-3)bDGalp(1-5)xXKdoa

SVGMWSMILES3D molIonize

 

O=C(O)C1(O)C[C@@H](O)[C@@H](O[C@@H]2O[C@H](CO)[C@H](O)[C@H](O[C@H]3O[C@H](CO)[C@H](O[C@@H]4O[C@H](CO)[C@H](O)[C@H](O)[C@H]4O)[C@H](O)[C@H]3O)[C@H]2O)[C@@H]([C@H](O)CO)O1

724.615 g/mol (C₂₆H₄₄O₂₃)