Taxonomic group: bacteria / Chlamydiae
(Phylum: Chlamydiae)
Associated disease: infection due to Chlamydia [ICD11:
XN27H 
]
NCBI PubMed ID: 14625588Journal NLM ID: 9421566Publisher: Nature Publishing Co.
Correspondence: svevans
uvic.ca
Institutions: Institute of Chemistry, University of Agriculture, A-1190 Vienna, Austria, Department of Biochemistry, Microbiology and Immunology, University of Ottawa, 451 Smyth Road, Ottawa, Ontario K1H 8M5, Canada, National Research Council of Canada, Institute for Biological Sciences, 100 Sussex Drive, Ottawa, Ontario K1A 0R6, Canada, Division of Medical and Biochemical Microbiology, Research Center Borstel, Center for Medicine and Biosciences, Parkallee 1-40, D-23845 Borstel, Germany, Department of Biochemistry and Microbiology, University of Victoria, PO Box 3800, STN CSC, Victoria, British Columbia V8W 3P6, Canada.
High-resolution structures reveal how a germline antibody can recognize a range of clinically relevant carbohydrate epitopes. The germline response to a carbohydrate immunogen can be critical to survivability, with selection for antibody gene segments that both confer protection against common pathogens and retain the flexibility to adapt to new disease organisms. We show here that antibody S25-2 binds several distinct inner-core epitopes of bacterial lipopolysaccharides (LPSs) by linking an inherited monosaccharide residue binding site with a subset of complementarity-determining regions (CDRs) of limited flexibility positioned to recognize the remainder of an array of different epitopes. This strategy allows germline antibodies to adapt to different epitopes while minimizing entropic penalties associated with the immobilization of labile CDRs upon binding of antigen, and provides insight into the link between the genetic origin of individual CDRs and their respective roles in antigen recognition
Lipopolysaccharide, genetic, antigen, lipopolysaccharides, structure, common, disease, gene, Bacterial, role, carbohydrate, antibodies, antibody, epitope, recognition, response, epitopes, region, inner core, pathogen, pathogens, binding, binding site, site, monosaccharide, protection, high-resolution, flexibility, origin, high resolution, immobilization, immunogen, labile, selection
Structure type: oligomer
Location inside paper: p. 1020, fig. 1a
Trivial name: disaccharide a-(2-8)-linked Kdo
Compound class: LPS
Contained glycoepitopes: IEDB_130650,IEDB_130658
Biological activity: binding with S25-2 antibody
Comments, role: inner-core epitope found in enterobacterial genera
3D data: conformation data
Related record ID(s): 565, 3235, 3881, 4143, 4921, 22428, 116695
NCBI Taxonomy refs (TaxIDs): 810Reference(s) to other database(s): GlycomeDB:
5769
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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