Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
The structure was elucidated in this paperJournal NLM ID: 0043535WWW link: http://www.sciencedirect.com/science/article/pii/S0008621501003007Publisher: Elsevier
Correspondence: vito.ferro
progen.com.au
Institutions: Department of Research & Development, Progen Industries Ltd, PO Box 28, Richlands BC, Qld 4077, Australia
The promising new anticancer agent, PI-88, is prepared by the sulfonation of the oligosaccharide phosphate fraction of the extracellular phosphomannan produced by the yeast Pichia (Hansenula) holstii NRRL Y-2448. The composition of the oligosaccharide phosphate fraction was determined by capillary electrophoresis (CE) with indirect UV detection using 6 mM potassium sorbate at pH 10.3 as the background electrolyte. Further confirmation of the composition was obtained by HPLC analysis of a sample dephosphorylated by treatment with alkaline phosphatase. The structure of the hexasaccharide component has been determined by isolation and NMR spectroscopic analysis of its dephosphorylated derivative. Additionally, the structure of a second, previously undetected tetrasaccharide component (a hexosamine) has been determined by isolation and NMR spectroscopic analysis of the acetate of its dephosphorylated derivative. It is demonstrated that CE is an ideal method for the quality control of the oligosaccharide phosphate fraction for use in the production of PI-88.
capillary electrophoresis, Pichia (Hansenula) holstii, phosphomannan, oligosaccharide phosphate fraction, PI-88
Structure type: oligomer
Location inside paper: p. 144, structure 6
Compound class: EPS
Contained glycoepitopes: IEDB_130701,IEDB_136104,IEDB_137485,IEDB_140116,IEDB_141111,IEDB_143632,IEDB_144983,IEDB_152206,IEDB_164174,IEDB_164176,IEDB_983930,SB_136,SB_196,SB_197,SB_44,SB_67,SB_72
Methods: 13C NMR, 1H NMR, GC-MS, dephosphorylation, TLC, ESI-MS, methanolysis, HPLC, UV, capillary electrophoresis (CE), methylation analysis, CC, HMBC, HMQC, COSY, NOESY, ion chromatography, capillary electrophoresis (CE) (CE)
Related record ID(s): 40438, 40599, 40600, 40601, 40602, 40604, 40605, 40606, 40607, 40608, 40609, 40610
NCBI Taxonomy refs (TaxIDs): 36025Reference(s) to other database(s): GTC:G80699WY
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There is only one chemically distinct structure:
Found 
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(later renamed to: Nakazawaea holstii NRRL Y-2448)