Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
The structure was elucidated in this paperPublication DOI: 10.1016/S0008-6215(99)00332-8Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: jd
crc.dk
Institutions: Department of Chemistry, Carlsberg Laboratory, Gamle Carlsberg Vej 10, DK-2500 Valby, Copenhagen, Denmark
Mannans from genetically modified Pichia pastoris yeast, used for overproduction of neural cell adhesion molecule protein, grown on normal media or on uniformly 13C-labeled glucose and methanol, were isolated and characterized by high-field (750 MHz) NMR spectroscopy. Fully 13C-labeled oligosaccharide fragments were prepared from mannans by acetolysis. According to the data obtained, the mannan is made up of a main chain of α-(1→6)-linked mannopyranosyl residues, substituted at O-2 with α-mannopyranosyl or a α-d-Manp-(1→2)-β-D-Manp-(1→2)-β-D-Manp-(1→2)-α-d-Manp- group, and with much lower content of substitution with β-D-Manp-(1→2)-α-d-Manp-. A fraction of these oligosaccharide side chains is again substituted with α-d-Glcp or α-d-GlcpNAc through a phosphodiester linkage to the 6 position of the first mannopyranosyl residue. Improved conditions of acetolysis, cleaving all α-(1→6) linkages, but not β-mannoside linkages, are proposed.
yeast, Pichia pastoris, mannan; 13C labeled
Structure type: oligomer
Location inside paper: p. 220 (structure 2)
Trivial name: mannobiose
Compound class: EPS, mannan
Contained glycoepitopes: IEDB_130701,IEDB_136104,IEDB_137485,IEDB_143632,IEDB_144983,IEDB_152206,IEDB_983930,SB_136,SB_196,SB_44,SB_67,SB_72
Methods: 13C NMR, 1H NMR, gel filtration, 31P NMR, acetolysis, TOCSY, DQF-COSY, NOESY, HSQC HSQC–TOCSY
Related record ID(s): 40453, 40641, 40643, 40838
NCBI Taxonomy refs (TaxIDs): 4922Reference(s) to other database(s): GTC:G29313LW
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There is only one chemically distinct structure:
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(later renamed to: Komagataella pastoris His 4 GS-115)