Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
Organ / tissue: cell wallAssociated disease: infection due to Aspergillus fumigatus [ICD11:
XN5Z7 
]
The structure was elucidated in this paperPublication DOI: 10.1074/jbc.M909975199Journal NLM ID: 2985121RWWW link: http://www.jbc.org/content/275/36/27594.abstractPublisher: Baltimore, MD: American Society for Biochemistry and Molecular Biology
Correspondence: Thierry Fontaine <tfontain
pasteur.fr>
Institutions: Laboratoire des Aspergillus, Institut Pasteur, 25 rue du Docteur Roux, 75724 Paris cedex 15, France, Laboratoire de Résonance Magnétique Nucléaire, Institut Pasteur, 28 rue du Docteur Roux, 75724 Paris cedex 15, France, Laboratoire de Chimie Biologique, Universitédes Sciences et Technologie de Lille Flandres-Artois 59655 Villeneuve d'Ascq cedex, France, University of Athens, Department of Biology, Division of Biochemistry and Molecular Biology GR-15701, Athens, Greece
Physical and biological properties of the fungal cell wall are determined by the composition and arrangement of the structural polysaccharides. Cell wall polymers of fungi are classically divided into two groups depending on their solubility in hot alkali. We have analyzed the alkali-insoluble fraction of the Aspergillus fumigatus cell wall, which is the fraction believed to be responsible for fungal cell wall rigidity. Using enzymatic digestions with recombinant endo-β-1,3-glucanase and chitinase, fractionation by gel filtration, affinity chromatography with immobilized lectins, and high performance liquid chromatography, several fractions that contained specific interpolysaccharide covalent linkages were isolated. Unique features of the A. fumigatuscell wall are (i) the absence of β-1,6-glucan and (ii) the presence of a linear β-1,3/1,4-glucan, never previously described in fungi. Galactomannan, chitin, and β-1,3-glucan were also found in the alkali-insoluble fraction. The β-1,3-glucan is a branched polymer with 4% of β-1,6 branch points. Chitin, galactomannan, and the linear β-1,3/1,4-glucan were covalently linked to the nonreducing end of β-1,3-glucan side chains. As in Saccharomyces cerevisiae, chitin was linked via a β-1,4 linkage to β-1,3-glucan. The data obtained suggested that the branching of β-1,3-glucan is an early event in the construction of the cell wall, resulting in an increase of potential acceptor sites for chitin, galactomannan, and the linear β-1,3/1,4-glucan.
Structure type: structural motif or average structure
Location inside paper: p. 27598, structure 2 (QzSN IIe)
Contained glycoepitopes: IEDB_114708,IEDB_1397514,IEDB_141806,IEDB_142488,IEDB_146664,IEDB_153543,IEDB_158555,IEDB_161166,IEDB_2278476,IEDB_2278477,IEDB_241101,IEDB_558869,IEDB_857743,IEDB_983931,SB_192
Methods: 13C NMR, 1H NMR, GLC-MS, gel filtration, acid hydrolysis, GLC, mild acid hydrolysis, HPAEC, enzymatic digestion, 15N NMR, acetolysis, TOCSY, methylation analysis, DQF-COSY, MALDI-TOF-MS, phenol-sulfuric acid procedure, Johnson procedure, lectin affinity chromatography, gHSQC-TOCSY
Comments, role: analytical product; a chain between branching and reducing end (exclusively) and a chain attached to position 6 may have both from 1 to 3 bGlc; a chain at position 6 may have 4 or 5 bGlc; total number of bGlc is 10. To provide NMR data, the structure is given with maximal length of every chain. NMR temperature was 304-313
Related record ID(s): 40488, 40704, 40705, 40706
NCBI Taxonomy refs (TaxIDs): 746128Reference(s) to other database(s): GTC:G06263ZF
Show glycosyltransferases
NMR conditions: in D2O at 308(H) K
[as TSV]
13C NMR data:
missing...
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
3,3,3,3,3,3,3 b?Glc? 4.76 3.37 3.53 3.415 3.49 3.72-3.92
3,3,3,3,3,3 b?Glc? 4.80 3.56 3.79 3.53 3.52 3.75-3.93
3,3,3,3,3 b?Glc? 4.80 3.56 3.79 3.53 3.52 3.75-3.93
3,3,3,3,6,3,3,3,3,3 b?Glc? 4.76 3.37 3.53 3.415 3.49 3.72-3.92
3,3,3,3,6,3,3,3,3 b?Glc? 4.80 3.56 3.79 3.53 3.52 3.75-3.93
3,3,3,3,6,3,3,3 b?Glc? 4.80 3.56 3.79 3.53 3.52 3.75-3.93
3,3,3,3,6,3,3 b?Glc? 4.80 3.56 3.79 3.53 3.52 3.75-3.93
3,3,3,3,6,3 b?Glc? 4.77 3.56 3.79 3.53 3.52 3.75-3.93
3,3,3,3,6 b?Glc? 4.56 3.52 3.75 3.52 3.52 3.75-3.93
3,3,3,3 b?Glc? 4.77 3.58 3.80 3.61 3.70 3.90-4.22
3,3,3 b?Glc? 4.80 3.56 3.79 3.53 3.52 3.75-3.93
3,3 b?Glc? 4.80 3.56 3.79 3.53 3.52 3.75-3.93
3 b?Glc? 4.68 3.59 3.79 3.53 3.52 3.75-3.93
x?Glc-ol 3.68-3.79 4.03 4.06 3.66 3.91 3.655-3.86
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 3,3,3,3,3,3,3 | b?Glc? | 4.76 | 3.37 | 3.53 | 3.415 | 3.49 | 3.72
3.92 |
| 3,3,3,3,3,3 | b?Glc? | 4.80 | 3.56 | 3.79 | 3.53 | 3.52 | 3.75
3.93 |
| 3,3,3,3,3 | b?Glc? | 4.80 | 3.56 | 3.79 | 3.53 | 3.52 | 3.75
3.93 |
| 3,3,3,3,6,3,3,3,3,3 | b?Glc? | 4.76 | 3.37 | 3.53 | 3.415 | 3.49 | 3.72
3.92 |
| 3,3,3,3,6,3,3,3,3 | b?Glc? | 4.80 | 3.56 | 3.79 | 3.53 | 3.52 | 3.75
3.93 |
| 3,3,3,3,6,3,3,3 | b?Glc? | 4.80 | 3.56 | 3.79 | 3.53 | 3.52 | 3.75
3.93 |
| 3,3,3,3,6,3,3 | b?Glc? | 4.80 | 3.56 | 3.79 | 3.53 | 3.52 | 3.75
3.93 |
| 3,3,3,3,6,3 | b?Glc? | 4.77 | 3.56 | 3.79 | 3.53 | 3.52 | 3.75
3.93 |
| 3,3,3,3,6 | b?Glc? | 4.56 | 3.52 | 3.75 | 3.52 | 3.52 | 3.75
3.93 |
| 3,3,3,3 | b?Glc? | 4.77 | 3.58 | 3.80 | 3.61 | 3.70 | 3.90
4.22 |
| 3,3,3 | b?Glc? | 4.80 | 3.56 | 3.79 | 3.53 | 3.52 | 3.75
3.93 |
| 3,3 | b?Glc? | 4.80 | 3.56 | 3.79 | 3.53 | 3.52 | 3.75
3.93 |
| 3 | b?Glc? | 4.68 | 3.59 | 3.79 | 3.53 | 3.52 | 3.75
3.93 |
| | x?Glc-ol | 3.68
3.79 | 4.03 | 4.06 | 3.66 | 3.91 | 3.655
3.86 |
|
There are too many chemically distinct structures (~134217728), so only one is shown:
bDGlcp(1-3)bDGlcp(1-3)bDGlcp(1-3)[bDGlcp(1-3)bDGlcp(1-3)bDGlcp(1-3)bDGlcp(1-3)bDGlcp(1-3)bDGlcp(1-6)]bDGlcp(1-3)bDGlcp(1-3)bDGlcp(1-3)bDGlcp(1-3)xDGlc-ol
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Found 1 record.
Displayed record 1
