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Taxonomic group: fungi / Basidiomycota (Phylum: Basidiomycota)
Associated disease: infection due to Cryptococcus neoformans [ICD11: XN3EH

]

Publication DOI: 10.1073/pnas.211229198
Journal NLM ID: 7505876
Publisher: National Academy of Sciences
Correspondence: peled

ccrc.uga.edu
Institutions: Complex Carbohydrate Research Center and Department of Botany, University of Georgia, Athens, GA 30602, USA, Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, MO 63110, USA

UDP-xylose is a sugar donor required for the synthesis of diverse and important glycan structures in animals, plants, fungi, and bacteria. Xylose-containing glycans are particularly abundant in plants and in the polysaccharide capsule that is the major virulence factor of the pathogenic fungus Cryptococcus neoformans. Biosynthesis of UDP-xylose is mediated by UDP-glucuronic acid decarboxylase, which converts UDP-glucuronic acid to UDP-xylose. Although this enzymatic activity was described over 40 years ago it has never been fully purified, and the gene encoding it has not been identified. We used homology to a bacterial gene, hypothesized to encode a related function, to identify a cryptococcal sequence as putatively encoding a UDP-glucuronic acid decarboxylase. A soluble 47-kDa protein derived from bacteria expressing the C. neoformans gene catalyzed conversion of UDP-glucuronic acid to UDP-xylose, as confirmed by NMR analysis. NADH, UDP, and UDP-xylose inhibit the activity. Close homologs of the cryptococcal gene, which we termed UXS1, appear in genome sequence data from organisms ranging from bacteria to humans.

Structure type: polymer chemical repeating unit
Location inside paper: fig. 1, GalXM
Contained glycoepitopes: IEDB_114701,IEDB_130701,IEDB_134624,IEDB_136044,IEDB_136095,IEDB_136906,IEDB_137472,IEDB_1394182,IEDB_141794,IEDB_144983,IEDB_145668,IEDB_151528,IEDB_152206,IEDB_164174,IEDB_167188,IEDB_174332,IEDB_190606,IEDB_983930,SB_163,SB_165,SB_166,SB_187,SB_195,SB_197,SB_44,SB_67,SB_7,SB_72,SB_88

Methods: 1H NMR, SDS-PAGE, HPLC, cloning, protein expression, UDP-GlcA decarboxylase assay

Related record ID(s): 40538
NCBI Taxonomy refs (TaxIDs): 5207
Reference(s) to other database(s): GTC:G36303MW
Show glycosyltransferases

There are too many chemically distinct structures (~67108864), so only one is shown:

-6)aDGalp(1-6)[bDXylp(1-3)[bDXylp(1-2)[bDXylp(1-3)aDManp(1-3)]aDManp(1-4)]bDGalp(1-3)]aDGalp(1-6)aDGalp(1-6)[aDManp(1-3)aDManp(1-4)bDGalp(1-3)]aDGalp(1-

*OC[C@H]1O[C@H](OC[C@H]2O[C@H](OC[C@H]3O[C@H](OC[C@H]4O[C@H](*)[C@H](O)[C@@H](O[C@@H]5O[C@H](CO)[C@H](O[C@H]6O[C@H](CO)[C@@H](O)[C@H](O[C@H]7O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]7O)[C@@H]6O)[C@H](O)[C@H]5O)[C@H]4O)[C@H](O)[C@@H](O)[C@H]3O)[C@H](O)[C@@H](O[C@@H]3O[C@H](CO)[C@H](O[C@H]4O[C@H](CO)[C@@H](O)[C@H](O[C@H]5O[C@H](CO)[C@@H](O)[C@H](O[C@@H]6OC[C@@H](O)[C@H](O)[C@H]6O)[C@@H]5O)[C@@H]4O[C@@H]4OC[C@@H](O)[C@H](O)[C@H]4O)[C@H](O[C@@H]4OC[C@@H](O)[C@H](O)[C@H]4O)[C@H]3O)[C@H]2O)[C@H](O)[C@@H](O)[C@H]1O

SVG MW SMILES 3D mol Ionize