Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
Organ / tissue: mycelium
NCBI PubMed ID: 34944419Publication DOI: 10.3390/biom11121777Journal NLM ID: 101596414Publisher: Basel, Switzerland: MDPI
Correspondence: Roszczyk A <aleksander.roszczyk
wum.edu.pl>; Zych M <michal.zych
wum.edu.pl>; Kniotek M <monika.kniotek
wum.edu.pl>; Zagożdżon R <radoslaw.zagozdzon
wum.edu.pl>; Klimaszewska M <marzenna.klimaszewska
wum.edu.pl>; Malinowska E <eliza.malinowska
wum.edu.pl>; Turło J <jadwiga.turlo
wum.edu.pl>; Pac M <michal.pac
student.wum.edu.pl>; Kaleta B <beata.kaleta
wum.edu.pl>
Institutions: Department of Drug Technology and Pharmaceutical Biotechnology, Medical University of Warsaw, Warsaw, Poland, Department of Clinical Immunology, Medical University of Warsaw, Warsaw, Poland, Department of Immunology, Transplantology, and Internal Diseases, Medical University of Warsaw, Warsaw, Poland
A common edible mushroom Lentinula edodes, is an important source of numerous biologically active substances, including polysaccharides, with immunomodulatory and antitumor properties. In the present work, the biological activity of the crude, homogenous (Se)-enriched fraction (named Se-Le-30), which has been isolated from L. edodes mycelium by a modified Chihara method towards human peripheral blood mononuclear cells (PBMCs) and peripheral granulocytes, was investigated. The Se-Le-30 fraction, an analog of lentinan, significantly inhibited the proliferation of human PBMCs stimulated with anti-CD3 antibodies or allostimulated, and down-regulated the production of tumor necrosis factor (TNF)-? by CD3+ T cells. Moreover, it was found that Se-Le-30 significantly reduced the cytotoxic activity of human natural killer (NK) cells. The results suggested the selective immunosuppressive activity of this fraction, which is non-typical for mushroom derived polysaccharides.
polysaccharides, Lentinula edodes, selenium, immunosuppressant
Structure type: homopolymer
Location inside paper: Supplementary S1, C
Trivial name: glucan, β-1,3-glucan, curdlan, curdlan-type polysaccharide 13140, paramylon, curdlan, laminarin, β-glucan, curdlan, β-(1,3)-glucan, β-(1,3)-glucan, curdlan, curdlan, β-1,3-glucan, paramylon, reserve polysaccharide, b-glucan, β-1,3-D-glucan, laminaran, botryosphaeran, laminaran type β-D-glucan, latiglucan I, pachymaran, Curdlan, zymosan A, β-glucan, curdlan, laminarin, zymosan, zymosan, glucan particles, zymosan, β-(1-3)-glucan, β-(1,3)-glucan, β-(1,3)glucan, pachymaran, D-glucan (DPn)540, pachyman, laminaran, curdlan, zymosan, zymosan, β-(1,3)-glucan, zymosan A, zymosan, β-1,3-glucan, curdlan, β-1,3-glucan, curdlan, β-1,3-glucan, curdlan, pachyman, β-(1,3)-glucan, curdlan, callose, a water-insoluble β-(1→3)-glucan, fermentum β-polysaccharide, water-insoluble glucan, alkali-soluble β-glucan (PeA3), alkali-soluble polysaccharide (PCAP), callose, laminarin
Compound class: EPS, O-polysaccharide, cell wall polysaccharide, lipophosphoglycan, glycoprotein, LPG, glucan, polysaccharide, glycoside, β-glucan, β3-glucan, cell wall glucan
Contained glycoepitopes: IEDB_1397514,IEDB_142488,IEDB_146664,IEDB_153543,IEDB_158555,IEDB_161166,IEDB_2278476,IEDB_2278477,IEDB_558869,IEDB_857743,IEDB_983931,SB_192
Methods: biological assays, extraction, RP-HPLC, cell growth, cytokine production, cytotoxicity assay, Bradford method, ROS measurement, fluorometry
Related record ID(s): 40976, 40977, 40979, 40980
NCBI Taxonomy refs (TaxIDs): 5353Reference(s) to other database(s): GTC:G51056AN, GlycomeDB:
157, CCSD:
50049, CBank-STR:4225, CA-RN: 51052-65-4, GenDB:FJ3380871.1
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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