Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
Organ / tissue: mycelia
NCBI PubMed ID: 37925437Publication DOI: 10.1038/s41467-023-42693-6Journal NLM ID: 101528555Publisher: London: Nature Publishing Group
Correspondence: R.A. Batista-García <rabg
uaem.mx>; T. Wang <wangtuo1
msu.edu>
Institutions: Department of Chemistry, Michigan State University, East Lansing, MI, USA, Complex Carbohydrate Research Center, University of Georgia, Athens, GA, 30602, USA, Centro de Investigación en Dinámica Celular, Universidad Autónoma del Estado de Morelos, Cuernavaca, Mexico, Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, WA, USA, Department of Biology, University of Ljubljana, Ljubljana, Slovenia, Institute of Molecular Biology and Biotechnology, University of Crete, Heraklion, Greece, Fungal Respiratory Infections Research Unit, University of Angers, Angers, France
Halophilic fungi thrive in hypersaline habitats and face a range of extreme conditions. These fungal species have gained considerable attention due to their potential applications in harsh industrial processes, such as bioremediation and fermentation under unfavorable conditions of hypersalinity, low water activity, and extreme pH. However, the role of the cell wall in surviving these environmental conditions remains unclear. Here we employ solid-state NMR spectroscopy to compare the cell wall architecture of Aspergillus sydowii across salinity gradients. Analyses of intact cells reveal that A. sydowii cell walls contain a rigid core comprising chitin, β-glucan, and chitosan, shielded by a surface shell composed of galactomannan and galactosaminogalactan. When exposed to hypersaline conditions, A. sydowii enhances chitin biosynthesis and incorporates α-glucan to create thick, stiff, and hydrophobic cell walls. Such structural rearrangements enable the fungus to adapt to both hypersaline and salt-deprived conditions, providing a robust mechanism for withstanding external stress. These molecular principles can aid in the optimization of halophilic strains for biotechnology applications.
cell wall, solid-state NMR, Aspergillus sydowii, halophilic fungi
Structure type: homopolymer
Location inside paper: Fig. 1a, chitin
Trivial name: chitin
Compound class: O-polysaccharide, cell wall polysaccharide, glucan, polysaccharide, chitin
Contained glycoepitopes: IEDB_135813,IEDB_137340,IEDB_141807,IEDB_151531,IEDB_153212,IEDB_241099,IEDB_423114,IEDB_423150,SB_74,SB_85
Methods: isotopic labeling, TEM, ssNMR, culture conditions, ssNMR-2D
Related record ID(s): 41408, 41409, 41410, 41411, 41412
NCBI Taxonomy refs (TaxIDs): 75750Reference(s) to other database(s): GTC:G97099AY, CCSD:
46067, CBank-STR:5851, GenDB:KF905651
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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