Polysaccharides α-D-galactan (GAL-Am) and β-D-glucan (GLC-Am) were obtained from Amanita muscaria fruiting bodies. They were purified using different methodologies, such as Fehling precipitation (for both fractions), freeze-thawing process and ultrafiltration (for GLC-Am). Results showed that the GAL-Am has (1→6)-linked Galp main chain branched at O-2 by terminal Galp units and has not been previously reported. Besides, GLC-Am has (1→3)-linked Glcp in the main chain, substituted at O-6 by (1→6)-linked β-Glcp units. Both are water-soluble, with 9.0 × 103 g/moL and 1.3 × 105 g/moL, respectively. GAL-Am and GLC-Am presented a selective proliferation reduction against B16-F10 melanoma cell line, not affecting non tumoral BALB/3T3 fibroblast cell line. Furthermore, both fractions reduced clonogenic capacity of melanoma cell line over an extended period of time. These results were obtained without modulations in B16-F10 cell adhesion, reinforcing the biological activities towards cell proliferation impairment and eliciting these polysaccharides as promising compounds to further exploration of their antimelanoma properties.

chemical structure, polysaccharides, β-glucan, β-D-glucan, α-galactan, A. muscaria, antimelanoma properties

13C NMR data:
Linkage	Residue	C1	C2	C3	C4	C5	C6
	bDGlcp	103.0	72.9	86.2	68.4	76.4	60.9


1H NMR data:
Linkage	Residue	H1	H2	H3	H4	H5	H6
	bDGlcp	4.53	3.31	3.49	3.25	3.27	3.48-3.71


1H/13C HSQC data:
Linkage	Residue	C1/H1	C2/H2	C3/H3	C4/H4	C5/H5	C6/H6
	bDGlcp	103.0/4.53	72.9/3.31	86.2/3.49	68.4/3.25	76.4/3.27	60.9/3.48-3.71

There is only one chemically distinct structure:

SVGMWSMILES3D molIonize

 

*O[C@@H]1[C@@H](O)[C@H](*)O[C@H](CO)[C@H]1O

162.141 g/mol (C₆H₁₀R₂O₅, R = next and previous repeats, not counted in mol weight)