Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
The structure was elucidated in this paperPublication DOI: 10.1016/S0008-6215(03)00146-0Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: Yoshio Okawa <okawa
tohoku-pharm.ac.jp>
Institutions: Second Department of Hygienic Chemistry, Tohoku Pharmaceutical University, 4-4-1 Komatsushima, Sendai Aoba-ku, Miyagi 981-8558, Japan
Torulaspora delbrueckii starin IFO 0955 was examined with respect to its structural and serological properties of the cell wall mannan (Td-0955-M). Td-0955-M revealed significant reactivities with sera from a commercially available factor serum kit (Candida Check) in ELISA. Td-0955-M was investigated for its chemical structure by acetolysis under conventional and mild conditions. NMR and GC techniques were used as analytical techniques. The mannooligosaccharide fractions eluted from a Bio-Gel P-2 column were found to consist of Man(α1-2)Man, M2, Man(α1-2)Man(α1-2)Man and Man(β1-2)Man(α1-2)Man, M3, Man(α1-2)Man(β1-2)Man(β1-2)Man(α1-2)Man, M5, and a new mannoheptaose, which possesses the structure, Man(α1-2)Man(β1-2)Man(β1-2)Man(β1-2)Man(β1-2)Man(α1-2)Man, M7. The results of the inhibition ELISA showed that the M7 oligosaccharide significantly inhibited the reactivities in the Td-0955-M-factor serum systems.
serology, mannan, antigenic epitopes, yeast, Torulaspora delbrueckii, Manno-oligosaccharides
Structure type: oligomer
Location inside paper: abstract, p.1177, table 1, oligosaccharide M2
Trivial name: mannobiose
Compound class: EPS, mannan
Contained glycoepitopes: IEDB_130701,IEDB_136104,IEDB_137485,IEDB_143632,IEDB_144983,IEDB_152206,IEDB_983930,SB_136,SB_196,SB_44,SB_67,SB_72
Methods: 1H NMR, methylation, ELISA, GC, chemical methods, serological methods, GPC, acetolysis, GFC, HOHAHA
Biological activity: serological data
Comments, role: The major oligosaccharide obtained by acetolysis of mannan fraction Td-0955-M (from the Torulaspora delbrueckii strain IFO 0955) under mild conditions.
Related record ID(s): 41203, 41204, 41205, 41206, 41207, 41389
NCBI Taxonomy refs (TaxIDs): 4950Reference(s) to other database(s): GTC:G29313LW
Show glycosyltransferases
NMR conditions: in D2O at 318(H) K
[as TSV]
13C NMR data:
missing...
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
2 aDManp 5.039 ? ? ? ? ?
?DManp 5.367 ? ? ? ? ?
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 2 | aDManp | 5.039 | ? | ? | ? | ? | ? |
| | ?DManp | 5.367 | ? | ? | ? | ? | ? |
|
There is only one chemically distinct structure:
Found 
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