Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
Organ / tissue: mycelium
The structure was elucidated in this paperNCBI PubMed ID: 12829396Publication DOI: 10.1016/S0008-6215(03)00197-6Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: lnzhang
public.wh.hb.cn
Institutions: Department of Chemistry, Wuhan University, Wuhan 430072, PR China, Department of Biology, The Chinese University of Hong Kong, Hong Kong, The Laboratory of Applied Mycology, Huazhong Agricultural University, Wuhan 430070, PR China
Mycelia of a wild strain Poria cocos were cultured in two media differing in one constituent: bran extract or corn steep liquor, and are designated as wb and wc, respectively. Six polysaccharide fractions were isolated sequentially from the two mycelia by 0.9% NaCl (PCM1), hot water (PCM2), 0.5 M NaOH (PCM3-I and -II) and 88% formic acid (PCM4-I and -II). Their chemical and physical characteristics were determined by infrared spectroscopy (IR), gas chromatography (GC), 13C NMR, light scattering (LS) and viscometry. The results indicated that wb-, wc-PCM1, and PCM2 were heteropolysaccharides mainly composed of α-d-glucose, mannose, and galactose, whereas wb-PCM3-I and wc-PCM3-I were mainly (1→3)-α-d-glucans, and wb- and wc-PCM3-II, PCM4-I and PCM4-II were (1→3)-β-D-glucans. Interestingly, (1→3) α- and (1→3)-β-D-glucans co-existed in the 0.5 M NaOH fraction and were separated individually into the two fractions (PCM3-I and PCM3-II) after neutralizing with acetic acid. The polysaccharides from wc-PCM cultured in media containing corn steep liquor contained relatively more protein. The polysaccharide fractions also existed in conformations including random coil (as in PCM0 and PCM1) and expanded chain (as in PCM3), and differed molecular mass. In addition, two exo-polysaccharides isolated from the two culture media by methanol precipitation (wb- and wc-PCM0) also differed in their monosaccharide composition.
conformation, polysaccharide, light scattering, composition, molecular mass, Poria cocos
Structure type: homopolymer
Location inside paper: abstract, p.1513, fractions: PCM4-I, wb-PCM4-II, wb-PCM3-II, wc-PCM3-II
Trivial name: glucan, β-1,3-glucan, curdlan, curdlan-type polysaccharide 13140, paramylon, curdlan, laminarin, β-glucan, curdlan, β-(1,3)-glucan, β-(1,3)-glucan, curdlan, curdlan, β-1,3-glucan, paramylon, reserve polysaccharide, b-glucan, β-1,3-D-glucan, laminaran, botryosphaeran, laminaran type β-D-glucan, latiglucan I, pachymaran, Curdlan, zymosan A, β-glucan, curdlan, laminarin, zymosan, zymosan, glucan particles, zymosan, β-(1-3)-glucan, β-(1,3)-glucan, β-(1,3)glucan, pachymaran, D-glucan (DPn)540, pachyman, laminaran, curdlan, zymosan, zymosan, β-(1,3)-glucan, zymosan A, zymosan, β-1,3-glucan, curdlan, β-1,3-glucan, curdlan, β-1,3-glucan, curdlan, pachyman, β-(1,3)-glucan, curdlan, callose, a water-insoluble β-(1→3)-glucan, fermentum β-polysaccharide, water-insoluble glucan, alkali-soluble β-glucan (PeA3), alkali-soluble polysaccharide (PCAP), callose, laminarin
Compound class: EPS, O-polysaccharide, cell wall polysaccharide, lipophosphoglycan, glycoprotein, LPG, glucan, polysaccharide, glycoside, β-glucan, β3-glucan, cell wall glucan
Contained glycoepitopes: IEDB_1397514,IEDB_142488,IEDB_146664,IEDB_153543,IEDB_158555,IEDB_161166,IEDB_2278476,IEDB_2278477,IEDB_558869,IEDB_857743,IEDB_983931,SB_192
Methods: 13C NMR, IR, GC, light scattering, viscosity measurement, extraction, SEC
Comments, role: Mainly glucans; NMR data of wc-PCM3-II polysaccharide. NMR data for wb-PCM4-II polysaccharide: #_bDGlcp 103.0 72.8 86.3 68.4 76.3 60.9//.
Related record ID(s): 41212, 43701
NCBI Taxonomy refs (TaxIDs): 81056Reference(s) to other database(s): GTC:G51056AN, GlycomeDB:
157, CCSD:
50049, CBank-STR:4225, CA-RN: 51052-65-4, GenDB:FJ3380871.1
Show glycosyltransferases
NMR conditions: in DMSO-d6 at 295(C) K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
bDGlcp 103.0 72.9 86.2 68.5 76.3 60.9
1H NMR data:
missing...
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| | bDGlcp | 103.0 | 72.9 | 86.2 | 68.5 | 76.3 | 60.9 |
|
There is only one chemically distinct structure:
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Found 1 record.
Displayed record 1
(later renamed to: Wolfiporia cocos)