Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
Organ / tissue: sclerotium
The structure was elucidated in this paperNCBI PubMed ID: 14698891Publication DOI: 10.1016/j.carres.2003.10.006Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: lnzhang
public.wh.hb.cn
Institutions: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, Department of Chemistry, National University of Ireland, Galway, Ireland, Department of Chemistry, Wuhan University, Wuhan 430072, China
Six polysaccharides were extracted sequentially from the fresh sclerotium of Poria cocos cultivated in China using 0.9% NaCl (PCS1), hot water (PCS2), 0.5 M NaOH (PCS3-I and PCS3-II), and 88% formic acid (PCS4-I and PCS4-II). Their chemical and physical characteristics were determined using infrared spectroscopy (IR), gas chromatography (GC), GC–MS methylation analysis, 13C NMR spectroscopy, elementary analysis (EA), protein analysis, size exclusion chromatography combined with laser light scattering (SEC-LLS), light scattering (LS), and viscometry. The results indicated that the polysaccharides PCS1, PCS2, and PCS3-I were heteropolysaccharides containing d-glucose, d-galactose, d-mannose, d-fucose, and d-xylose; the predominant monosaccharide was d-glucose except for PCS1 where it was d-galactose. PCS3-II, the main component of the sclerotium of P. cocos, was a linear (1→3)-β-D-glucan of high purity. PCS4-I consisted of (1→3)-β-D-glucan with some β-(1→6) linked branches. PCS4-II was mainly composed of (1→3)-β-D-glucan containing some glucose branches. The Mw values of the six polysaccharides PCS1, PCS2, PCS3-I, PCS4-I in 0.2 M NaCl aqueous solution, PCS3-II, and PCS4-II in dimethyl sulfoxide (Me2SO) were determined to be 11.6 × 104, 20.8 × 104, 17.1 × 104, 9.1 × 104, 12.3 × 104, and 21.1 × 104, respectively. The six polysaccharides in aqueous solution or Me2SO exist as flexible chains. The chemical and physical characteristics of six polysaccharides isolated from the fresh sclerotium of Poria cocos were determined by IR, GC, GC–MS, 13C NMR, LLS, SEC-LLS, and viscometry. The Mw values ranged from 9.1 × 104 to 21.1 × 104. The six polysaccharides in aqueous solution or Me2SO exist as flexible chains.
polysaccharide, 13C NMR, light scattering, molecular mass, Poria cocos sclerotium, Chemical component
Structure type: homopolymer ; 123000
Location inside paper: abstract, p.332, table 2, PCS3-II
Trivial name: β-1,3-D-glucan
Compound class: glucan
Contained glycoepitopes: IEDB_1397514,IEDB_142488,IEDB_146664,IEDB_153543,IEDB_158555,IEDB_161166,IEDB_2278476,IEDB_2278477,IEDB_558869,IEDB_857743,IEDB_983931,SB_192
Methods: 13C NMR, methylation, IR, GC-MS, GC, light scattering, UV, viscosity measurement, extraction, SEC
Comments, role: The predominant component of polysaccharides isolated from the sclerotium of P. cocos, PCS3-II, was (1->3)-b-D-glucan.
Related record ID(s): 41218, 41219
NCBI Taxonomy refs (TaxIDs): 81056Reference(s) to other database(s): GTC:G51056AN
Show glycosyltransferases
NMR conditions: in D2O at 333(C) K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
bDGlcp 102.9 72.7 86.2 68.3 76.3 60.8
1H NMR data:
missing...
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| | bDGlcp | 102.9 | 72.7 | 86.2 | 68.3 | 76.3 | 60.8 |
|
There is only one chemically distinct structure:
Found 
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(later renamed to: Wolfiporia cocos)