Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
Organ / tissue: cell wall
The structure was elucidated in this paperNCBI PubMed ID: 15337454Publication DOI: 10.1016/j.carres.2004.05.033Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: Naohito Ohno <ohnonao
ps.toyaku.ac.jp>
Institutions: School of Pharmacy, Tokyo University of Pharmacy and Life Science, Tokyo, Japan, Cosmetic Laboratory, Kanebo Cosmetics, Inc., 5-3-28, Kotobuki-cho, Odawara-shi, Kanagawa 250-0002, Japan, Department of Chemical and Biological Sciences, Faculty of Science, Japan Women’s University, 2-8-1, Mejirodai, Bunkyo-ku, Tokyo 112-8681, Japan, Japan Women’s University, 2-8-1, Mejirodai, Bunkyo-ku, Tokyo 112-8681, Japan, Institute of Medical Mycology, Teikyo University, 539, Otsuka, Hachioji, Tokyo 192-0395, Japan
Alkali extraction and methylation analyses in the 1970s revealed that the cell walls of the yeast Schizosaccharomyces pombe contain a (1→3)-α-d-glucan, a (1→3)-β-D-glucan, a (1→6)-β-D-glucan, and a α-galactomannan. To refine the structures of these polysaccharides, cell-wall glucans of S. pombe were extracted, fractionated, and analyzed by NMR spectroscopy. S. pombe cells were treated with 3% NaOH, and alkali-soluble and insoluble fractions were prepared. The alkali-insoluble fraction was treated with 0.5 M acetic acid or Zymolyase 100 T to yield an alkali-insoluble, acetic acid-insoluble fraction, an alkali-insoluble, Zymolyase-insoluble fraction, and an alkali-insoluble, Zymolyase-soluble fraction. 13C NMR and 2D-NMR spectra disclosed that the cell wall of S. pombe is composed of three types of glucans, specifically, a (1→3)-α-d-glucan, a (1→3)-β-D-glucan, which may either be linear or slightly branched, and a highly branched (1→6)-β-D-glucan, in addition to α-galactomannan. The highly branched (1→6)-β-D-glucan was identified by selective periodate degradation of side-chain glucose as a highly (1→3)-β-branched (1→6)-β-D-glucan with more branches than that of Saccharomyces cerevisiae. Flexibility of these polysaccharides in the cell wall was analyzed by 13C NMR spectra in D2O. The data collectively indicate that (1→3)-α- and (1→3)-β-D-glucans are rigid and contribute to the cell shape, while the highly branched (1→6)-β-D-glucan and α-galactomannan are flexible.
NMR, cell-wall glucan, Schizosaccharomyces pombe
Structure type: homopolymer
Location inside paper: p.2256, fig.1, (c) fraction 3
Trivial name: glucan, β-1,3-glucan, curdlan, curdlan-type polysaccharide 13140, paramylon, curdlan, laminarin, β-glucan, curdlan, β-(1,3)-glucan, β-(1,3)-glucan, curdlan, curdlan, β-1,3-glucan, paramylon, reserve polysaccharide, b-glucan, β-1,3-D-glucan, laminaran, botryosphaeran, laminaran type β-D-glucan, latiglucan I, pachymaran, Curdlan, zymosan A, β-glucan, curdlan, laminarin, zymosan, zymosan, glucan particles, zymosan, β-(1-3)-glucan, β-(1,3)-glucan, β-(1,3)glucan, pachymaran, D-glucan (DPn)540, pachyman, laminaran, curdlan, zymosan, zymosan, β-(1,3)-glucan, zymosan A, zymosan, β-1,3-glucan, curdlan, β-1,3-glucan, curdlan, β-1,3-glucan, curdlan, pachyman, β-(1,3)-glucan, curdlan, callose, a water-insoluble β-(1→3)-glucan, fermentum β-polysaccharide, water-insoluble glucan, alkali-soluble β-glucan (PeA3), alkali-soluble polysaccharide (PCAP), callose, laminarin
Compound class: EPS, O-polysaccharide, cell wall polysaccharide, lipophosphoglycan, glycoprotein, LPG, glucan, polysaccharide, glycoside, β-glucan, β3-glucan, cell wall glucan
Contained glycoepitopes: IEDB_1397514,IEDB_142488,IEDB_146664,IEDB_153543,IEDB_158555,IEDB_161166,IEDB_2278476,IEDB_2278477,IEDB_558869,IEDB_857743,IEDB_983931,SB_192
Methods: 13C NMR, 1H NMR, methylation, gel filtration, NMR-2D, sugar analysis, acid hydrolysis, GLC, alkaline degradation, Smith degradation, MALDI-TOF MS, enzymatic digestion, extraction
Comments, role: The alkali-extraction, glucan of S. pombe contains a highly purifed (1-3)-β-D-glucan. The NMR assignment is missing from the paper, assigned by CSDB staff.
Related record ID(s): 41230, 41232, 42136
NCBI Taxonomy refs (TaxIDs): 4896Reference(s) to other database(s): GTC:G51056AN, GlycomeDB:
157, CCSD:
50049, CBank-STR:4225, CA-RN: 51052-65-4, GenDB:FJ3380871.1
Show glycosyltransferases
NMR conditions: in DMSO-d6 at 343(C) K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
bDGlcp 102.82 72.67 86.10 68.30 76.24 60.80
1H NMR data:
missing...
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| | bDGlcp | 102.82 | 72.67 | 86.10 | 68.30 | 76.24 | 60.80 |
|
There is only one chemically distinct structure:
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